Rabbit RPL27A Polyclonal Antibody | anti-RPL27A antibody

RPL27A, CT (RPL27A, 60S ribosomal protein L27a) (PE)

Cat. Num. AAA6343467

• Gene Names: RPL27A; L27A
• Reactivity: Hamster, Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RPL27A; Polyclonal Antibody; CT (RPL27A; 60S ribosomal protein L27a) (PE); 60S ribosomal protein L27a; anti-RPL27A antibody

• Host: Rabbit
• Reactivity: Hamster, Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-RPL27A antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: RPL27A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 113-142 amino acids from the C-terminal region of human RPL27A.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(RPL27A Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the RPL27A antibody detected the RPL27A protein (arrow).)

Western Blot (WB)

(RPL27A Antibody (C-term) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the RPL27A antibody detected the RPL27A protein (arrow).)

Immunohistochemistry (IHC)

(RPL27A Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RPL27A Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(RPL27A Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-RPL27A antibody

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L15P family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, multiple processed pseudogenes derived from this gene are dispersed through the genome.

Product Categories/Family for anti-RPL27A antibody

Antibodies; Ribosomal Proteins

References

Kapp, L.D., et al. Annu. Rev. Biochem. 73, 657-704 (2004): Mazumder, B., et al. Cell 115(2):187-198(2003). Andersen, J.S., et al. Curr. Biol. 12(1):1-11(2002). Bortoluzzi, S., et al. Bioinformatics 17(12):1152-1157(2001). Kusuda, J., et al. Cytogenet. Cell Genet. 85 (3-4), 248-251 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 4506625
• NCBI GeneID: 6157
• NCBI Accession #: NP_000981.1
• NCBI GenBank Nucleotide #: NM_000990.4
• UniProt Accession #: P46776; B2R4B3
• Molecular Weight: 16,561 Da
• NCBI Official Full Name: 60S ribosomal protein L27a
• NCBI Official Synonym Full Names: ribosomal protein L27a
• NCBI Official Symbol: RPL27A
• NCBI Official Synonym Symbols: L27A
• NCBI Protein Information: 60S ribosomal protein L27a
• UniProt Protein Name: 60S ribosomal protein L27a
• Protein Family: 60S ribosomal protein
• UniProt Gene Name: RPL27A
• UniProt Entry Name: RL27A_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L15P family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, multiple processed pseudogenes derived from this gene are dispersed through the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

RPL27A: a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L15P family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, multiple processed pseudogenes derived from this gene are dispersed through the genome. [provided by RefSeq, Jul 2008]

Protein type: Ribosomal; Translation

Chromosomal Location of Human Ortholog: 11p15

Cellular Component: membrane; cytosol

Molecular Function: protein binding; structural constituent of ribosome; RNA binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; translational elongation; cellular protein metabolic process; translation; viral reproduction; mRNA catabolic process, nonsense-mediated decay; translational initiation; gene expression; viral transcription; translational termination; viral infectious cycle