Rabbit RPL27A Polyclonal Antibody | anti-RPL27A antibody

RPL27A, CT (RPL27A, 60S ribosomal protein L27a)

Cat. Num. AAA6004058

• Gene Names: RPL27A; RPL27
• Reactivity: Hamster, Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: RPL27A; Polyclonal Antibody; CT (RPL27A; 60S ribosomal protein L27a); Anti -RPL27A; anti-RPL27A antibody

• Host: Rabbit
• Reactivity: Hamster, Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human, Hamster
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-RPL27A antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100; Flow Cytometry: 1:10-50
• Immunogen: RPL27A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 113-142 amino acids from the C-terminal region of human RPL27A.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RPL27A Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the RPL27A antibody detected the RPL27A protein (arrow).)

Western Blot (WB)

(RPL27A Antibody (C-term) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the RPL27A antibody detected the RPL27A protein (arrow).)

Immunohistochemistry (IHC)

(RPL27A Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RPL27A Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(RPL27A Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-RPL27A antibody

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L15P family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, multiple processed pseudogenes derived from this gene are dispersed through the genome.

Product Categories/Family for anti-RPL27A antibody

Antibodies; Abs to Ribosomal Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 6321798
• NCBI GeneID: 856401
• NCBI Accession #: NP_011874.1
• NCBI GenBank Nucleotide #: NM_001179140.1
• UniProt Accession #: P0C2H6; P38706; D3DKV5
• Molecular Weight: 15,531 Da
• NCBI Official Full Name: ribosomal 60S subunit protein L27A
• NCBI Official Symbol: RPL27A
• NCBI Official Synonym Symbols: RPL27
• NCBI Protein Information: ribosomal 60S subunit protein L27A
• UniProt Protein Name: 60S ribosomal protein L27-A
• Protein Family: 60S ribosomal protein
• UniProt Gene Name: RPL27A
• UniProt Synonym Gene Names: RPL27
• UniProt Entry Name: RL27A_YEAST

Uniprot Description

Subunit structure: Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.3

Subcellular location: Cytoplasm Ref.5.

Miscellaneous: Present with 7380 molecules/cell in log phase SD medium.There are 2 genes for L27 in yeast.

Sequence similarities: Belongs to the ribosomal protein L27e family.