Rabbit anti-Human NRG2 Polyclonal Antibody | anti-NRG2 antibody

NRG2, CT (Neuregulin 2, Pro-neuregulin-2, Membrane-bound Isoform, Pro-NRG2, Neuregulin-2, NTAK) (Biotin)

Cat. Num. AAA6489793

• Reactivity: Human
• Applications: Immunohistochemistry, ELISA
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: NRG2; Polyclonal Antibody; CT (Neuregulin 2; Pro-neuregulin-2; Membrane-bound Isoform; Pro-NRG2; Neuregulin-2; NTAK) (Biotin); anti-NRG2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NRG2.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-NRG2 antibody: Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptides between 366~396 amino acids from the C-terminal region (a portion within aa834-850) and center region (a portion within aa 373-389) from human NRG2, conjugated to KLH.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-NRG2 antibody

Antibodies; Growth Factors; Cytokines

References

1. Yamada, K., et al., Gene 255(1):15-24 (2000). 2. Ring, H.Z., et al., Hum. Genet. 104(4):326-332 (1999). 3. Busfield, S.J., et al., Mol. Cell. Biol. 17(7):4007-4014 (1997). 4. Higashiyama, S., et al., J. Biochem. 122(3):675-680 (1997). 5. Carraway, K.L. III, et al., Nature 387(6632):512-516 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 7669536
• UniProt Accession #: O14511
• Molecular Weight: 91.678
• UniProt Protein Name: Pro-neuregulin-2, membrane-bound isoform
• Protein Family: Pro-neuregulin
• UniProt Gene Name: NRG2
• UniProt Synonym Gene Names: NTAK; Pro-NRG2; NRG-2; DON-1; NTAK
• UniProt Entry Name: NRG2_HUMAN

Uniprot Description

NRG2: Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. May also promote the heterodimerization with the EGF receptor. Belongs to the neuregulin family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Ligand, receptor tyrosine kinase

Chromosomal Location of Human Ortholog: 5q31.2

Cellular Component: extracellular space; plasma membrane; integral to membrane; extracellular region

Molecular Function: growth factor activity; receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; embryonic development; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; organ development; innate immune response; signal transduction