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Western Blot (WB) (Western blot analysis of RPL17 expression in A375 (A), Raw264.7 (B), H9C2 (C) whole cell lysates.)

Rabbit RPL17 Polyclonal Antibody | anti-RPL17 antibody

Anti-RPL17 Antibody

Gene Names
RPL17; L17; PD-1; RPL23
Reactivity
Human, Mouse, Rat, Bovine, Monkey
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
RPL17; Polyclonal Antibody; Anti-RPL17 Antibody; 60S ribosomal protein L17; 60S ribosomal protein L23; PD-1; anti-RPL17 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Monkey
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of RPL17 protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Sequence Length
184
Applicable Applications for anti-RPL17 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes
WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RPL17. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of RPL17 expression in A375 (A), Raw264.7 (B), H9C2 (C) whole cell lysates.)

Western Blot (WB) (Western blot analysis of RPL17 expression in A375 (A), Raw264.7 (B), H9C2 (C) whole cell lysates.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of RPL17 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of RPL17 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Related Product Information for anti-RPL17 antibody
Rabbit polyclonal antibody to RPL17

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
26,373 Da
NCBI Official Full Name
60S ribosomal protein L17 isoform a
NCBI Official Synonym Full Names
ribosomal protein L17
NCBI Official Symbol
RPL17
NCBI Official Synonym Symbols
L17; PD-1; RPL23
NCBI Protein Information
60S ribosomal protein L17; 60S ribosomal protein L23; gene encoding putative NFkB activating protein
UniProt Protein Name
60S ribosomal protein L17
Protein Family
UniProt Gene Name
RPL17
UniProt Entry Name
RL17_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L22P family of ribosomal proteins. It is located in the cytoplasm. This gene has been referred to as rpL23 because the encoded protein shares amino acid identity with ribosomal protein L23 from Halobacterium marismortui; however, its official symbol is RPL17. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream C18orf32 (chromosome 18 open reading frame 32) gene. [provided by RefSeq, Dec 2010]

Uniprot Description

RPL17: a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L22P family of ribosomal proteins. It is located in the cytoplasm. This gene has been referred to as rpL23 because the encoded protein shares amino acid identity with ribosomal protein L23 from Halobacterium marismortui; however, its official symbol is RPL17. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream C18orf32 (chromosome 18 open reading frame 32) gene. [provided by RefSeq, Dec 2010]

Protein type: Ribosomal; Translation

Chromosomal Location of Human Ortholog: 18q21

Cellular Component: nucleus; cytosol

Molecular Function: structural constituent of ribosome

Biological Process: SRP-dependent cotranslational protein targeting to membrane; translational elongation; cellular protein metabolic process; viral reproduction; translation; mRNA catabolic process, nonsense-mediated decay; translational initiation; viral transcription; gene expression; translational termination; viral infectious cycle

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Product Notes

The RPL17 rpl17 (Catalog #AAA821214) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RPL17 Antibody reacts with Human, Mouse, Rat, Bovine, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's RPL17 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP). WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100). Researchers should empirically determine the suitability of the RPL17 rpl17 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPL17, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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