Chicken Colony Stimulating Factor 2 Receptor Alpha ELISA Kit | CSF2Ra elisa kit

Chicken Colony Stimulating Factor 2 Receptor Alpha ELISA Kit

Cat. Num. AAA752907

• Gene Names: CSF2RA; GMR; CD116; CSF2R; SMDP4; CDw116; CSF2RX; CSF2RY; GMCSFR; CSF2RAX; CSF2RAY; GM-CSF-R-alpha
• Reactivity: Chicken
• Synonyms: Colony Stimulating Factor 2 Receptor Alpha; Chicken Colony Stimulating Factor 2 Receptor Alpha ELISA Kit; CSF2Ra elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Intended Uses: This CSF2Ra ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken CSF2Ra. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This CSF2Ra ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken CSF2Ra. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CSF2Ra elisa kit

Principle of the Assay: CSF2Ra ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CSF2Ra antibody and an CSF2Ra-HRP conjugate. The assay sample and buffer are incubated together with CSF2Ra-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CSF2Ra concentration since CSF2Ra from samples and CSF2Ra-HRP conjugate compete for the anti-CSF2Ra antibody binding site. Since the number of sites is limited, as more sites are occupied by CSF2Ra from the sample, fewer sites are left to bind CSF2Ra-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CSF2Ra concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CSF2Ra elisa kit

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 47938316
• NCBI GeneID: 1438
• Molecular Weight: 31,101 Da
• NCBI Official Full Name: CSF2RA protein
• NCBI Official Synonym Full Names: colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage)
• NCBI Official Symbol: CSF2RA
• NCBI Official Synonym Symbols: GMR; CD116; CSF2R; SMDP4; CDw116; CSF2RX; CSF2RY; GMCSFR; CSF2RAX; CSF2RAY; GM-CSF-R-alpha
• NCBI Protein Information: granulocyte-macrophage colony-stimulating factor receptor subunit alpha; GMR-alpha; GMCSFR-alpha; CD116 antigen; GM-CSF receptor alpha subunit; colony stimulating factor 2 receptor alpha subunit; granulocyte-macrophage colony-stimulating factor receptor alpha chain
• UniProt Protein Name: Granulocyte-macrophage colony-stimulating factor receptor subunit alpha
• Protein Family: Granulocyte-macrophage colony-stimulating factor receptor
• UniProt Gene Name: CSF2RA
• UniProt Synonym Gene Names: CSF2R; CSF2RY; GM-CSF-R-alpha; GMCSFR-alpha; GMR-alpha
• UniProt Entry Name: CSF2R_HUMAN

NCBI Description

The protein encoded by this gene is the alpha subunit of the heterodimeric receptor for colony stimulating factor 2, a cytokine which controls the production, differentiation, and function of granulocytes and macrophages. The encoded protein is a member of the cytokine family of receptors. This gene is found in the pseudoautosomal region (PAR) of the X and Y chromosomes. Multiple transcript variants encoding different isoforms have been found for this gene, with some of the isoforms being membrane-bound and others being soluble. [provided by RefSeq, Jul 2008]

Uniprot Description

CSF2RA: Low affinity receptor for granulocyte-macrophage colony- stimulating factor. Transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells. Defects in CSF2RA are the cause of pulmonary surfactant metabolism dysfunction type 4 (SMDP4). A rare lung disorder due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress. Belongs to the type I cytokine receptor family. Type 5 subfamily. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: Xp22.32 and Yp11.3

Cellular Component: integral to plasma membrane; plasma membrane; extracellular region

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; receptor activity

Biological Process: response to ethanol; cytokine and chemokine mediated signaling pathway

Disease: Surfactant Metabolism Dysfunction, Pulmonary, 4