Western Blot (WB)
(Western blot analysis of RPC expression in HeLa (A), NIH3T3 (B), rat stomach (C) whole cell lysates.)
Rabbit RPC Polyclonal Antibody | anti-RTCA antibody
Anti-RPC Antibody
Western Blot (WB)
(Western blot analysis of RPC expression in HeLa (A), NIH3T3 (B), rat stomach (C) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of RPC expression in HeLa (A), NIH3T3 (B), rat stomach (C) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of RPC staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of RPC staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the RNA 3'-phosphate cyclase family. The encoded protein plays a role in RNA metabolism by catalyzing the ATP-dependent conversion of the 3'-phosphate of RNA substrates to a 2',3'-cyclic phosphodiester. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]
Uniprot Description
RTCD1: Catalyzes the conversion of 3'-phosphate to a 2',3'- cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA- N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'- phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing. Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Ligase; EC 6.5.1.4; RNA-binding
Chromosomal Location of Human Ortholog: 1p21.2
Cellular Component: nucleoplasm; nucleus
Molecular Function: RNA binding; RNA-3'-phosphate cyclase activity; ATP binding
Biological Process: RNA processing
Research Articles on RTCA
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Product Notes
The RTCA rtca (Catalog #AAA820971) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RPC Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RPC can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB (1/500 - 1/1000), IH (1/100 - 1/200). Researchers should empirically determine the suitability of the RTCA rtca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPC, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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