Rabbit anti-Human RPAP1 Polyclonal Antibody | anti-RPAP1 antibody

RPAP1, NT (RPAP1, KIAA1403, RNA polymerase II-associated protein 1) (FITC)

Cat. Num. AAA6343218

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RPAP1; Polyclonal Antibody; NT (RPAP1; KIAA1403; RNA polymerase II-associated protein 1) (FITC); RNA polymerase II-associated protein 1; anti-RPAP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-RPAP1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RPAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 281-310 amino acids from the N-terminal region of human RPAP1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RPAP1 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the RPAP1 antibody detected the RPAP1 protein (arrow).)

Related Product Information for anti-RPAP1 antibody

The function of this gene is unknown. [provided by RefSeq].

Product Categories/Family for anti-RPAP1 antibody

Antibodies; DNA Replication; Repair

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Kuraoka, I., et al. DNA Repair (Amst.) 6(6):841-851(2007). Ballif, B.A., et al. Mol. Cell Proteomics 3(11):1093-1101(2004). Ballif, B.A., et al. Mol. Cell Proteomics 3(11):1093-1101(2004). Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 24430139
• NCBI GeneID: 26015
• NCBI Accession #: NP_056355.2
• NCBI GenBank Nucleotide #: NM_015540.3
• UniProt Accession #: Q9BWH6; Q9H9I2; Q9NSQ5; Q9P2E4; Q9UFS7
• Molecular Weight: 84,735 Da
• NCBI Official Full Name: RNA polymerase II-associated protein 1
• NCBI Official Synonym Full Names: RNA polymerase II associated protein 1
• NCBI Official Symbol: RPAP1
• NCBI Protein Information: RNA polymerase II-associated protein 1
• UniProt Protein Name: RNA polymerase II-associated protein 1
• Protein Family: RNA polymerase II-associated protein
• UniProt Gene Name: RPAP1
• UniProt Synonym Gene Names: KIAA1403
• UniProt Entry Name: RPAP1_HUMAN

NCBI Description

This protein forms part of the RNA polymerase II (RNAPII) enzyme complex and may recruit RNAPII to chromatin through its interaction with acetylated histones. [provided by RefSeq, Jul 2012]

Uniprot Description

RPAP1: Forms an interface between the RNA polymerase II enzyme and chaperone/scaffolding protein, suggesting that it is required to connect RNA polymerase II to regulators of protein complex formation. Required for interaction of the RNA polymerase II complex with acetylated histone H3. Belongs to the RPAP1 family. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 15q15.1

Cellular Component: nucleus

Molecular Function: DNA binding; DNA-directed RNA polymerase activity

Biological Process: transcription from RNA polymerase II promoter