Western Blot (WB)
(Western blot analysis of SW620 cell lysate using GDF15 antibody.)
GDF15 Antibody | anti-GDF15 antibody
GDF15 Polyclonal Antibody
Gene Names
GDF15; PDF; MIC1; PLAB; MIC-1; NAG-1; PTGFB; GDF-15
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purification
Synonyms
GDF15; GDF15 Polyclonal Antibody; GDF-15; MIC-1; MIC1; NAG-1; PDF; PLAB; PTGFB; Growth/differentiation factor 15; Placental TGF-beta; Placental bone morphogenetic protein; Prostate differentiation factor; anti-GDF15 antibody
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Sequence Length
308
Applicable Applications for anti-GDF15 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
Recombinant protein of human GDF15
Species
Rabbit
Cross Reactivity
Human, Mouse, Rat
Preparation and Storage
Store at -20 degree C or -80 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of SW620 cell lysate using GDF15 antibody.)
Western Blot (WB)
(Western blot analysis of SW620 cell lysate using GDF15 antibody.)
Related Product Information for anti-GDF15 antibody
Macrophage inhibitory cytokine-1 (Mic-1), also termed GDF15 (1), PTGF-Beta (2), PLAB (3), PDF (4), and NAG-1 (5), is a divergent member of the transforming growth factor-Beta (TGF-Beta) superfamily (6). Like other family members, Mic-1 is synthesized as an inactive precursor that undergoes proteolytic processing involving removal of an N-terminal hydrophobic signal sequence followed by cleavage at a conserved RXXR site generating an active C-terminal domain that is secreted as a dimeric protein. Mic-1 is highly expressed in the placenta and is also dramatically increased by cellular stress, acute injury, inflammation, and cancer. In the brain, Mic-1 is found in the choroid plexus and is secreted into the cerebrospinal fluid (7). It is also a transcriptional target of the p53 tumor suppressor protein and may serve as a biomarker for p53 activity (8,9). During tumor progression, Mic-1 has various effects on apoptosis, differentiation, angiogenisis, and metastasis, and may also contribute to weight loss during cancer (10,11).
Product Categories/Family for anti-GDF15 antibody
References
1. Strelau, J. et al. (2000) J Neurosci 20, 8597-603. 2. Yokoyama-Kobayashi, M. et al. (1997) J Biochem 122, 622-6. 3. Hromas, R. et al. (1997) Biochim Biophys Acta 1354, 40-4. 4. Paralkar, V.M. et al. (1998) J Biol Chem 273, 13760-7. 5. Baek, S.J. et al. (2001) J Biol Chem 276, 33384-92. 6. Bootcov, M.R. et al. (1997) Proc Natl Acad Sci USA 94, 11514-9. 7. Strelau, J. et al. (2000) J Neural Transm Suppl, 273-6. 8. Kannan, K. et al. (2000) FEBS Lett 470, 77-82. 9. Yang, H. et al. (2003) Mol Cancer Ther 2, 1023-9. 10. Johnen, H. et al. (2007) Nat Med 13, 1333-40. 11. Bauskin, A.R. et al. (2006) Cancer Res 66, 4983-6.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34.15kDa
NCBI Official Full Name
growth/differentiation factor 15 preproprotein
NCBI Official Synonym Full Names
growth differentiation factor 15
NCBI Official Symbol
GDF15
NCBI Official Synonym Symbols
PDF; MIC1; PLAB; MIC-1; NAG-1; PTGFB; GDF-15
NCBI Protein Information
growth/differentiation factor 15
UniProt Protein Name
Growth/differentiation factor 15
Protein Family
UniProt Gene Name
GDF15
UniProt Synonym Gene Names
MIC1; PDF; PLAB; PTGFB; GDF-15; MIC-1; NAG-1; NRG-1
NCBI Description
This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. The protein is expressed in a broad range of cell types, acts as a pleiotropic cytokine and is involved in the stress response program of cells after cellular injury. Increased protein levels are associated with disease states such as tissue hypoxia, inflammation, acute injury and oxidative stress. [provided by RefSeq, Aug 2016]
Research Articles on GDF15
Similar Products
Product Notes
The GDF15 gdf15 (Catalog #AAA8503584) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's GDF15 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200 . Researchers should empirically determine the suitability of the GDF15 gdf15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GDF15, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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