Rabbit anti-Human ROR1 Polyclonal Antibody | anti-ROR1 antibody

ROR1 (ROR1, NTRKR1, Tyrosine-protein kinase transmembrane receptor ROR1, Neurotrophic tyrosine kinase, receptor-related 1) (MaxLight 650)

Cat. Num. AAA6343102

• Gene Names: ROR1; NTRKR1; dJ537F10.1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: ROR1; Polyclonal Antibody; ROR1 (ROR1; NTRKR1; Tyrosine-protein kinase transmembrane receptor ROR1; Neurotrophic tyrosine kinase; receptor-related 1) (MaxLight 650); receptor-related 1; anti-ROR1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ROR1. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-ROR1 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: IHC-P: Formalin fixed paraffin embedded. Requires HIER using sodium citrate buffer pH6.0 prior to staining.; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein mapping to a fragment of residues within amino acids 112-39 human ROR1.
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of ROR1 in 293 cell lysates (2ug/lane) using 041166. Lane 1: nontransfected Lane 2: transiently transfected with the ROR1 gene)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of formalin fixed and paraffin embedded human lung carcimona using 041166 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of 041166 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of NCI-H460 cells using 041166 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-ROR1 antibody

Antibodies; Transmembrane Proteins

References

1. Nomi, M., et al., Mol. Cell. Biol. 21(24):8329-8335 (2001). 2. Reddy, U.R., et al., Genomics 41(2):283-285 (1997). 3. Reddy, U.R., et al., Oncogene 13(7):1555-1559 (1996). 4. Masiakowski, P., et al., J. Biol. Chem. 267(36):26181-26190 (1992).

NCBI and Uniprot Product Information

• NCBI GI #: 118572711
• NCBI GeneID: 4919
• UniProt Accession #: Q01973; Q5VVX6; Q66K77; Q92776
• Molecular Weight: 937
• NCBI Official Full Name: Tyrosine-protein kinase transmembrane receptor ROR1
• NCBI Official Synonym Full Names: receptor tyrosine kinase-like orphan receptor 1
• NCBI Official Symbol: ROR1
• NCBI Official Synonym Symbols: NTRKR1; dJ537F10.1
• NCBI Protein Information: tyrosine-protein kinase transmembrane receptor ROR1; neurotrophic tyrosine kinase, receptor-related 1
• UniProt Protein Name: Tyrosine-protein kinase transmembrane receptor ROR1
• Protein Family: Inactive tyrosine-protein kinase transmembrane receptor
• UniProt Gene Name: ROR1
• UniProt Synonym Gene Names: NTRKR1
• UniProt Entry Name: ROR1_HUMAN

NCBI Description

This gene encodes a receptor tyrosine kinase-like orphan receptor that modulates neurite growth in the central nervous system. The encoded protein is a glycosylated type I membrane protein that belongs to the ROR subfamily of cell surface receptors. It is a pseudokinase that lacks catalytic activity and may interact with the non-canonical Wnt signalling pathway. This gene is highly expressed during early embryonic development but expressed at very low levels in adult tissues. Increased expression of this gene is associated with B-cell chronic lymphocytic leukaemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2012]

Uniprot Description

ROR1: Tyrosine-protein kinase receptor whose role is not yet clear. Expressed strongly in human heart, lung and kidney, but weakly in the CNS. Isoform Short is strongly expressed in fetal and adult CNS and in a variety of human cancers, including those originating from CNS or PNS neuroectoderm. Belongs to the protein kinase superfamily. Tyr protein kinase family. ROR subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.10.1; Protein kinase, tyrosine (receptor); Membrane protein, integral; Protein kinase, TK; Kinase, protein; TK group; Ror family

Chromosomal Location of Human Ortholog: 1p31.3

Cellular Component: integral to plasma membrane; cytoplasm; plasma membrane; receptor complex

Molecular Function: Wnt-protein binding; protein binding; transmembrane receptor protein tyrosine kinase activity; ATP binding

Biological Process: peptidyl-tyrosine phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway