Rat Disabled Homolog 1 (DAB1) ELISA Kit | DAB1 elisa kit

Rat Disabled Homolog 1 (DAB1) ELISA Kit

Cat. Num. AAA2023069

• Reactivity: Rat
• Synonyms: Disabled Homolog 1 (DAB1); Rat Disabled Homolog 1 (DAB1) ELISA Kit; DAB1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Disabled Homolog 1 (DAB1).; No significant cross-reactivity or interference between Disabled Homolog 1 (DAB1) and analogues was observed.
• Sequence Length: 555

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.107ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Disabled Homolog 1 (DAB1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Disabled Homolog 1 (DAB1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DAB1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Disabled Homolog 1 (DAB1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Disabled Homolog 1 (DAB1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Disabled Homolog 1 (DAB1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Disabled Homolog 1 (DAB1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for DAB1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 33350928
• NCBI GeneID: 1600
• NCBI Accession #: NP_066566.3
• NCBI GenBank Nucleotide #: NM_021080.3
• UniProt Accession #: O75553; Q4LE59; Q5T6M6; Q5T6M9; Q5T835; Q5T836; Q5T837; Q6NWS9; Q6NWT0; Q6NWT1; A4FU90; B3KTG3
• Molecular Weight: 59,950 Da
• NCBI Official Full Name: disabled homolog 1
• NCBI Official Synonym Full Names: Dab, reelin signal transducer, homolog 1 (Drosophila)
• NCBI Official Symbol: DAB1
• NCBI Protein Information: disabled homolog 1
• UniProt Protein Name: Disabled homolog 1
• Protein Family: Disabled
• UniProt Gene Name: DAB1
• UniProt Entry Name: DAB1_HUMAN

NCBI Description

The laminar organization of multiple neuronal types in the cerebral cortex is required for normal cognitive function. In mice, the disabled-1 gene plays a central role in brain development, directing the migration of cortical neurons past previously formed neurons to reach their proper layer. This gene is similar to disabled-1, and the protein encoded by this gene is thought to be a signal transducer that interacts with protein kinase pathways to regulate neuronal positioning in the developing brain. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

DAB1: an adaptor molecule functioning in neural development. The laminar organization of multiple neuronal types in the cerebral cortex is required for normal cognitive function. In mice, the disabled-1 gene plays a central role in brain development, directing the migration of cortical neurons past previously formed neurons to reach their proper layer. Thought to be a signal transducer that interacts with protein kinase pathways to regulate neuronal positioning in the developing brain. Associates with the SH2 domains of Src, Fyn and Abl. Phosphorylated in response to reelin induction in embryonic neurons

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1p32-p31

Cellular Component: neuron projection; membrane; cell soma; apical part of cell; perinuclear region of cytoplasm; postsynaptic density; cytosol; brush border

Molecular Function: phosphoinositide 3-kinase binding

Biological Process: cell-cell adhesion involved in neuronal-glial interactions involved in cerebral cortex radial glia guided migration; response to drug; cerebellum structural organization; Golgi localization; ventral spinal cord development; negative regulation of cell adhesion; adult walking behavior; radial glia guided migration of Purkinje cell; small GTPase mediated signal transduction; positive regulation of protein kinase activity; negative regulation of astrocyte differentiation; dendrite development; midgut development; cerebral cortex radially oriented cell migration; negative regulation of axonogenesis; positive regulation of neuron differentiation; negative regulation of JAK-STAT cascade