Goat ROBO3 Polyclonal Antibody | anti-ROBO3 antibody

ROBO3 (Roundabout Homolog 3, Roundabout-like Protein 3)

Cat. Num. AAA645389

• Gene Names: ROBO3; HGPS; RIG1; HGPPS; RBIG1
• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: ROBO3; Polyclonal Antibody; ROBO3 (Roundabout Homolog 3; Roundabout-like Protein 3); Anti -ROBO3 (Roundabout Homolog 3; anti-ROBO3 antibody

• Host: Goat
• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat
• Clonality: Polyclonal
• Specificity: Recognizes human ROBO3. Species sequence homology: Bovine, canine, mouse, porcine and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

• Applicable Applications for anti-ROBO3 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:32,000; Western Blot: 0.5-1ug/ml, observed in human brain, cerebellum and hippocampus, lysates on ~150kD bands
• Immunogen: Synthetic peptide corresponding to C-RRKQRKELSHYT, from human ROBO3, at the internal region of the protein (NP_071765.2).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot staining of human brain (cerebellum) lysate (35ug protein in RIPA buffer) using MBS645389 (0.5ug/ml). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-ROBO3 antibody

Roundabouts (ROBO) are cell-surface receptors that mediate repulsive signaling mechanisms at the central nervous system midline. However, ROBOs may also mediate attraction mechanisms in the context of vascular development.

Product Categories/Family for anti-ROBO3 antibody

Antibodies; Abs to Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 48476182
• NCBI GeneID: 64221
• NCBI Accession #: NP_071765.2
• NCBI GenBank Nucleotide #: NM_022370.3
• UniProt Accession #: Q96MS0
• Molecular Weight: 148,209 Da
• NCBI Official Full Name: roundabout homolog 3
• NCBI Official Synonym Full Names: roundabout, axon guidance receptor, homolog 3 (Drosophila)
• NCBI Official Symbol: ROBO3
• NCBI Official Synonym Symbols: HGPS; RIG1; HGPPS; RBIG1
• NCBI Protein Information: roundabout homolog 3; roundabout-like protein 3; retinoblastoma inhibiting gene 1
• UniProt Protein Name: Roundabout homolog 3
• Protein Family: Roundabout
• UniProt Gene Name: ROBO3
• UniProt Entry Name: ROBO3_HUMAN

NCBI Description

This gene is a member of the Roundabout (ROBO) gene family that controls neurite outgrowth, growth cone guidance, and axon fasciculation. ROBO proteins are a subfamily of the immunoglobulin transmembrane receptor superfamily. SLIT proteins 1-3, a family of secreted chemorepellants, are ligands for ROBO proteins and SLIT/ROBO interactions regulate myogenesis, leukocyte migration, kidney morphogenesis, angiogenesis, and vasculogenesis in addition to neurogenesis. This gene, ROBO3, has a putative extracellular domain with five immunoglobulin (Ig)-like loops and three fibronectin (Fn) type III motifs, a transmembrane segment, and a cytoplasmic tail with three conserved signaling motifs: CC0, CC2, and CC3 (CC for conserved cytoplasmic). Unlike other ROBO family members, ROBO3 lacks motif CC1. The ROBO3 gene regulates axonal navigation at the ventral midline of the neural tube. In mouse, loss of Robo3 results in a complete failure of commissural axons to cross the midline throughout the spinal cord and the hindbrain. Mutations ROBO3 result in horizontal gaze palsy with progressive scoliosis (HGPPS); an autosomal recessive disorder characterized by congenital absence of horizontal gaze, progressive scoliosis, and failure of the corticospinal and somatosensory axon tracts to cross the midline in the medulla. Alternative transcript variants have been described but have not been experimentally validated. [provided by RefSeq, Dec 2009]

Uniprot Description

ROBO3: Thought to be involved during neural development in axonal navigation at the ventral midline of the neural tube. In spinal chord development plays a role in guiding commissural axons probably by preventing premature sensitivity to Slit proteins thus inhibiting Slit signaling through ROBO1. Required for hindbrain axon midline crossing. Defects in ROBO3 are a cause of familial horizontal gaze palsy with progressive scoliosis (HGPPS). Patients show a medulla where motor and sensory projections appear uncrossed. Belongs to the immunoglobulin superfamily. ROBO family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q24.2

Cellular Component: axon; integral to membrane

Biological Process: axon guidance; neuron migration; axon midline choice point recognition

Disease: Gaze Palsy, Familial Horizontal, With Progressive Scoliosis