Rabbit anti-Human RNH1 Polyclonal Antibody | anti-RNH1 antibody

Anti-RNH1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8104115

• Gene Names: RNH1; RAI; RNH
• Reactivity: Human
• Applications: Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: RNH1; Polyclonal Antibody; Anti-RNH1 Antibody; Rabbit Polyclonal; RNH1 Antibody; Rabbit PAb; Antigen Affinity Purified; ribonuclease/angiogenin inhibitor 1; Anti-RAI Antibody; Anti-RNH Antibody; anti-RNH1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human RNH1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-RNH1 antibody: Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: IHC-P: 1:500-1:3000; ICC/IF: 1:300-1:10,000
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human RNH1
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human RNH1, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human RNH1 in human testis with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human RNH1 in human brain with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.)

Immunofluorescence (IF)

(Immunofluorescence staining of RNH1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human RNH1 polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)

Related Product Information for anti-RNH1 antibody

Ribonuclease inhibitor (RNH1) is a cytosolic protein that binds with femtomolar affinity to human ribonuclease 1 (RNase 1) and homologous secretory ribonucleases. RNH1 contains 32 cysteine residues and has been implicated as an antioxidant. RNH1 is a leucine-rich repeat domains-containing protein that binds to and inhibits ribonucleases. RNH1 attenuates the activity of IRE1 by interacting with its ribonuclease domain. The Crithidia fasciculata RNH1 gene encodes an RNase H, an enzyme that specifically degrades the RNA strand of RNA-DNA hybrids. The RNH1 gene is contained within an open reading frame (ORF) predicted to encode a protein of 53.7 kDa. Major active forms of RNase H (38 and 45 kDa) were observed on activity gels of extracts of wild-type cells or cells in which one allele of RNH1 was disrupted. Both forms of the C.fasciculata RNase H are encoded by the RNH1 gene.

References

Thomas SP, et al. (2016) Knockout of the ribonuclease inhibitor gene leaves human cells vulnerable to secretory ribonucleases. Biochemistry 55 (46): 6359-6362.Tavernier Q, et al. (2018) Regulation of ire1 rnase activity by the ribonuclease inhibitor 1 (rnh1). Cell Cycle 17 (15): 1901-1916.Ray DS, et al. (1995) Disruption of the crithidia fasciculata rnh1 gene results in the loss of two active forms of ribonuclease h. Nucleic Acids Res 23 (13): 2526-2530.Engel ML, et al. (2001) The crithidia fasciculata rnh1 gene encodes both nuclear and mitochondrial isoforms of rnase h. Nucleic Acids Res 29 (3): 725-731.

NCBI and Uniprot Product Information

• NCBI GI #: 42822874
• NCBI GeneID: 6050
• NCBI Accession #: NP_976319.1
• NCBI GenBank Nucleotide #: NM_203385.1
• UniProt Accession #: P13489; Q8IZK8; Q96FD7; Q9BQ80; Q9UDK6
• Molecular Weight: 49,973 Da
• NCBI Official Full Name: ribonuclease inhibitor
• NCBI Official Synonym Full Names: ribonuclease/angiogenin inhibitor 1
• NCBI Official Symbol: RNH1
• NCBI Official Synonym Symbols: RAI; RNH
• NCBI Protein Information: ribonuclease inhibitor; placental RNase inhibitor; placental ribonuclease inhibitor
• UniProt Protein Name: Ribonuclease inhibitor
• Protein Family: Ribonuclease inhibitor
• UniProt Gene Name: RNH1
• UniProt Synonym Gene Names: PRI; RNH; Placental RNase inhibitor; RAI
• UniProt Entry Name: RINI_HUMAN

NCBI Description

Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular RNases (summarized by Lee et al., 1988 [PubMed 3219362]). In addition to control of intracellular RNases, the inhibitor may have a role in the regulation of angiogenin (MIM 105850). Ribonuclease inhibitor, of 50,000 Da, binds to ribonucleases and holds them in a latent form. Since neutral and alkaline ribonucleases probably play a critical role in the turnover of RNA in eukaryotic cells, RNH may be essential for control of mRNA turnover; the interaction of eukaryotic cells with ribonuclease may be reversible in vivo.[supplied by OMIM, Jul 2010]

Uniprot Description

RNH1: Ribonuclease inhibitor which inhibits RNASE1, RNASE2 and ANG. May play a role in redox homeostasis.

Protein type: Inhibitor

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: angiogenin-PRI complex; cytoplasm

Molecular Function: protein binding; ribonuclease inhibitor activity

Biological Process: negative regulation of catalytic activity; mRNA catabolic process; regulation of angiogenesis