Rabbit anti-Human UBA7 Polyclonal Antibody | anti-UBA7 antibody

UBA7 Antibody

Cat. Num. AAA9200769

• Gene Names: UBA7; D8; UBE2; UBA1B; UBE1L
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: UBA7; Polyclonal Antibody; UBA7 Antibody; Ubiquitin-like modifier-activating enzyme 7; Ubiquitin-activating enzyme 7; D8; Ubiquitin-activating enzyme E1 homolog; UBE1L; UBE2; anti-UBA7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: 0.01M PBS, pH 7.2, 0.1% Sodium azide, Glycerol 50%
• Concentration: 1mg/ml (varies by lot)
• Sequence Positions: 951-1010
• Sequence Length: 1012

• Applicable Applications for anti-UBA7 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)-Paraffin
• Application Notes: WB~~ 1:1000
• Antigen Type: synthetic peptide
• Antigen Source: KLH conjugated synthetic peptide derived from human UBA7
• Preparation and Storage: Store at -20 degree C.Stable for 12 months from date of receipt

Western Blot (WB)

(Anti-UBA7 Antibodyat 1:1000 dilution + Hela whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 112 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-UBA7 antibody

Activates ubiquitin by first adenylating with ATP its C- terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin- E1 thioester and free AMP. Catalyzes the ISGylation of influenza A virus NS1 protein.

Product Categories/Family for anti-UBA7 antibody

Cancer; Cell biology

References

Kok K.,et al.Proc. Natl. Acad. Sci. U.S.A. 90:6071-6075(1993).
Kok K.,et al.Gene Expr. 4:163-175(1995).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Gevaert K.,et al.Nat. Biotechnol. 21:566-569(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 38045948
• NCBI GeneID: 7318
• NCBI Accession #: NP_003326.2
• NCBI GenBank Nucleotide #: NM_003335.2
• UniProt Accession #: P41226; Q9BRB2
• Molecular Weight: 111694
• NCBI Official Full Name: ubiquitin-like modifier-activating enzyme 7
• NCBI Official Synonym Full Names: ubiquitin-like modifier activating enzyme 7
• NCBI Official Symbol: UBA7
• NCBI Official Synonym Symbols: D8; UBE2; UBA1B; UBE1L
• NCBI Protein Information: ubiquitin-like modifier-activating enzyme 7
• UniProt Protein Name: Ubiquitin-like modifier-activating enzyme 7
• Protein Family: Ubiquitin-like modifier-activating enzyme
• UniProt Gene Name: UBA7
• UniProt Synonym Gene Names: UBE1L; UBE2; Ubiquitin-activating enzyme 7
• UniProt Entry Name: UBA7_HUMAN

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme is a retinoid target that triggers promyelocytic leukemia (PML)/retinoic acid receptor alpha (RARalpha) degradation and apoptosis in acute promyelocytic leukemia, where it is involved in the conjugation of the ubiquitin-like interferon-stimulated gene 15 protein. [provided by RefSeq, Jul 2008]

Uniprot Description

UBE1L: Activates ubiquitin by first adenylating with ATP its C- terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin- E1 thioester and free AMP. Belongs to the ubiquitin-activating E1 family.

Protein type: Ubiquitin ligase; EC 6.3.2.19; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 3p21

Cellular Component: cytosol; nucleus

Molecular Function: ISG15 activating enzyme activity; ubiquitin-protein ligase activity; ubiquitin activating enzyme activity; ATP binding

Biological Process: modification-dependent protein catabolic process; ISG15-protein conjugation; cytokine and chemokine mediated signaling pathway; protein ubiquitination; protein modification process; innate immune response; negative regulation of interferon type I production