Rabbit anti-Human RNF43 Polyclonal Antibody | anti-RNF43 antibody

RNF43 Antibody

Cat. Num. AAA9414465

• Gene Names: RNF43; URCC; RNF124
• Reactivity: Human
• Applications: Western Blot
• Purity: Antigen affinity purification.
• Synonyms: RNF43; Polyclonal Antibody; RNF43 Antibody; URCC; RNF124; anti-RNF43 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total RNF43 protein.
• Purity/Purification: Antigen affinity purification.
• Form/Format: Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
• Concentration: 2.6 mg/ml (varies by lot)
• Sequence Length: 783

• Applicable Applications for anti-RNF43 antibody: Western Blot (WB)
• Application Notes: Western blotting: 1:200-1:1000
• Immunogen Type: Peptide
• Immunogen Description: Synthetic peptide corresponding to a region derived from internal residues of human ring finger protein 43
• SDS-PAGE MW: 86kD
• Target Name: RNF43
• Preparation and Storage: Store at -20°C

Testing Data

(Gel: 6%SDS-PAGELysate: 40ug Jurkat cellPrimary antibody: 1/300 dilution Secondary antibody dilution: 1/8000)

Related Product Information for anti-RNF43 antibody

RNF43 (ring finger protein 43), also known as URCC or RNF124, is a 783 amino acid single-pass type I membrane protein of the Endoplasmic reticulum and nuclear envelope that is thought to function as an E3 ubiquitin-protein ligase. Containing one RING-type zinc finger and existing as four alternatively spliced isoforms, RNF43 is expressed in fetal kidney and lung, as well as in cancers of the lung, colon and kidney. RNF43 functions as a cytotoxic T lymphocyte tumor antigen, and is therefore considered a target for cancer immunotherapy.

Product Categories/Family for anti-RNF43 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 770075665
• NCBI GeneID: 54894
• NCBI Accession #: NP_001292473.1
• NCBI GenBank Nucleotide #: NM_001305544.1
• UniProt Accession #: Q68DV7; Q65ZA4; Q6AI04; Q9NXD0; A8K4R2; B7Z443; B7Z5D5; B7Z5J5
• NCBI Official Full Name: E3 ubiquitin-protein ligase RNF43 isoform 1
• NCBI Official Synonym Full Names: ring finger protein 43
• NCBI Official Symbol: RNF43
• NCBI Official Synonym Symbols: URCC; RNF124
• NCBI Protein Information: E3 ubiquitin-protein ligase RNF43
• UniProt Protein Name: E3 ubiquitin-protein ligase RNF43
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: RNF43
• UniProt Entry Name: RNF43_HUMAN

NCBI Description

The protein encoded by this gene is a RING-type E3 ubiquitin ligase and is predicted to contain a transmembrane domain, a protease-associated domain, an ectodomain, and a cytoplasmic RING domain. This protein is thought to negatively regulate Wnt signaling, and expression of this gene results in an increase in ubiquitination of frizzled receptors, an alteration in their subcellular distribution, resulting in reduced surface levels of these receptors. Mutations in this gene have been reported in multiple tumor cells, including colorectal and endometrial cancers. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015]

Uniprot Description

RNF43: E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination, endocytosis and subsequent degradation of Wnt receptor complex components Frizzled. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone. Belongs to the ZNRF3 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; EC 6.3.2.-; Membrane protein, integral; Ubiquitin ligase; Ligase

Chromosomal Location of Human Ortholog: 17q22

Cellular Component: endoplasmic reticulum membrane; integral to plasma membrane; plasma membrane; nuclear envelope

Molecular Function: protein binding; zinc ion binding; frizzled binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: negative regulation of Wnt receptor signaling pathway; Wnt receptor signaling pathway; protein ubiquitination during ubiquitin-dependent protein catabolic process; protein ubiquitination