Testing Data
(Gel: 8%SDS-PAGE Lysates (from left to right): HepG2 and 293T cell Amount of lysate: 40ug per lane Primary antibody: 1/1400 dilution Secondary antibody dilution: 1/8000 Exposure time: 15seconds)
Rabbit anti-Human IFT74 Polyclonal Antibody | anti-IFT74 antibody
IFT74 Antibody
Gene Names
IFT74; CMG1; CCDC2; CMG-1
Reactivity
Human
Applications
Western Blot
Purity
Antigen affinity purification.
Synonyms
IFT74; Polyclonal Antibody; IFT74 Antibody; CMG1; CCDC2; CMG-1; anti-IFT74 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total IFT74 protein.
Purity/Purification
Antigen affinity purification.
Form/Format
Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
Concentration
2.8 mg/ml (varies by lot)
Sequence Length
600
Applicable Applications for anti-IFT74 antibody
Western Blot (WB)
Application Notes
Western blotting: 1:500-1:2000
Immunogen Type
Peptide
Immunogen Description
Synthetic peptide corresponding to a region derived from internal residues of human intraflagellar transport 74 homolog (Chlamydomonas)
Target Name
IFT74
Preparation and Storage
Store at -20 degree C
Testing Data
(Gel: 8%SDS-PAGE Lysates (from left to right): HepG2 and 293T cell Amount of lysate: 40ug per lane Primary antibody: 1/1400 dilution Secondary antibody dilution: 1/8000 Exposure time: 15seconds)
Testing Data
(Gel: 8%SDS-PAGE Lysates (from left to right): HepG2 and 293T cell Amount of lysate: 40ug per lane Primary antibody: 1/1400 dilution Secondary antibody dilution: 1/8000 Exposure time: 15seconds)
Related Product Information for anti-IFT74 antibody
Capillary morphogenesis protein 1 (CMG1), also known as intraflagellar transport protein 74 (ITP74) or coiled-coil domain-containing protein 2 (CCDC2), is a 600 amino acid human homologue of IFT-71, a complex B protein supporting intraflagellar transport (IFT) in Chlamydomonas. CMG1 localizes to the cytoplasmic vesicle and is highly expressed in adult and fetal kidney and testis, with lower levels of expression in adult heart, placenta, lung, liver and pancreas, and in fetal heart, lung and liver. CMG1 has been suggested to have a role in the primary cilia of HUVEC, and it also functions as a transcriptional regulator of cyclin D2 in spermatocyte-derived cells.
Product Categories/Family for anti-IFT74 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,332 Da
NCBI Official Full Name
intraflagellar transport protein 74 homolog isoform a
NCBI Official Synonym Full Names
intraflagellar transport 74
NCBI Official Symbol
IFT74
NCBI Official Synonym Symbols
CMG1; CCDC2; CMG-1
NCBI Protein Information
intraflagellar transport protein 74 homolog
UniProt Protein Name
Intraflagellar transport protein 74 homolog
Protein Family
UniProt Gene Name
IFT74
UniProt Synonym Gene Names
CCDC2; CMG1; CMG-1
UniProt Entry Name
IFT74_HUMAN
Uniprot Description
IFT74: 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Vesicle
Chromosomal Location of Human Ortholog: 9p21.2
Cellular Component: centrosome; cytoplasmic membrane-bound vesicle; nucleus; cilium
Molecular Function: beta-tubulin binding; chromatin binding
Biological Process: Notch signaling pathway; organelle organization and biogenesis; positive regulation of cell adhesion mediated by integrin; cilium biogenesis; positive regulation of transcription from RNA polymerase II promoter; negative regulation of epithelial cell proliferation
Research Articles on IFT74
Similar Products
Product Notes
The IFT74 ift74 (Catalog #AAA9414645) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IFT74 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IFT74 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western blotting: 1:500-1:2000. Researchers should empirically determine the suitability of the IFT74 ift74 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IFT74, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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