Western Blot (WB)
(RNASE1 Antibody (N-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the RNASE1 antibody detected the RNASE1 protein (arrow).)
Rabbit anti-Human RNASE1 Polyclonal Antibody | anti-RNASE1 antibody
RNASE1, NT (RNASE1, RIB1, RNS1, Ribonuclease pancreatic, HP-RNase, RIB-1, RNase UpI-1, Ribonuclease 1, Ribonuclease A)
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
RNASE1; Polyclonal Antibody; NT (RNASE1; RIB1; RNS1; Ribonuclease pancreatic; HP-RNase; RIB-1; RNase UpI-1; Ribonuclease 1; Ribonuclease A); Anti -RNASE1; anti-RNASE1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-RNASE1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunogen
RNASE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-42 amino acids from the N-terminal region of human RNASE1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(RNASE1 Antibody (N-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the RNASE1 antibody detected the RNASE1 protein (arrow).)
Western Blot (WB)
(RNASE1 Antibody (N-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the RNASE1 antibody detected the RNASE1 protein (arrow).)
Related Product Information for anti-RNASE1 antibody
This gene encodes a member of the pancreatic-type of secretory ribonucleases, a subset of the ribonuclease A superfamily. The encoded endonuclease cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substrate and hydrolyzes 2',3'-cyclic nucleotides, with a pH optimum near 8.0. The encoded protein is monomeric and more commonly acts to degrade ds-RNA over ss-RNA. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq].
Product Categories/Family for anti-RNASE1 antibody
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
17,644 Da
NCBI Official Full Name
RNASE1
UniProt Protein Name
Ribonuclease pancreatic
Protein Family
UniProt Gene Name
RNASE1
UniProt Synonym Gene Names
RIB1; RNS1; RNase 1; RNase A
UniProt Entry Name
RNAS1_HUMAN
Uniprot Description
Function: Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single-stranded and double-stranded RNA. Ref.19
Catalytic activity: Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.
Subunit structure: Monomer. Interacts with and forms tight 1:1 complexes with RNH1. Dimerization of two such complexes may occur. Interaction with RNH1 inhibits this protein. Ref.19
Subcellular location: Secreted.
Tissue specificity: Pancreas and other tissues and body fluids (indicating it may have other physiological functions besides its role in digestion).
Post-translational modification: N-linked glycans are of complex type.
Sequence similarities: Belongs to the pancreatic ribonuclease family.
Similar Products
Product Notes
The RNASE1 rnase1 (Catalog #AAA649922) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RNASE1, NT (RNASE1, RIB1, RNS1, Ribonuclease pancreatic, HP-RNase, RIB-1, RNase UpI-1, Ribonuclease 1, Ribonuclease A) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RNASE1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the RNASE1 rnase1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNASE1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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