Rabbit anti-Human MCTP2 Polyclonal Antibody | anti-MCTP2 antibody

Anti-MCTP2 Antibody, Rabbit Polyclonal

Cat. Num. AAA8109294

• Reactivity: Human
• Applications: Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: MCTP2; Polyclonal Antibody; Anti-MCTP2 Antibody; Rabbit Polyclonal; MCTP2 Antibody; Rabbit PAb; Antigen Affinity Purified; multiple C2 domains; transmembrane 2; anti-MCTP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human MCTP2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-MCTP2 antibody: Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: ICC/IF: 1:100-1:500
• Immunogen: E Coli-derived Human MCTP2 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human MCTP2 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Immunofluorescence (IF)

(Immunofluorescence staining of MCTP2 in RT4 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human MCTP2 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm.)

Related Product Information for anti-MCTP2 antibody

The invertebrate organisms Caenorhabditis elegans and Drosophila melanogaster express a single MCTP gene, whereas vertebrates express two MCTP genes (MCTP1 and MCTP2) whose primary transcripts are extensively alternatively spliced. Most of the MCTP sequences, in particular the C2 domains, are highly conserved. All MCTP C2 domains except for the second C2 domain of MCTP2 include a perfect Ca2+/phospholipid-binding consensus sequence. In higher eukaryotes, multiple C2 domain-containing transmembrane protein (MCTP2) is similar to Pex30: it contains an RHD and resides in ER domains where most nascent LD biogenesis occurs and that often associate with peroxisomes. The MCTP2 gene, located on chromosome 15q26, influences adiposity. The MCTP2 gene is involved in intercellular signal transduction and synapse function. MCTP2 is also a novel genetic cause of CoA and related cardiac malformations.

References

Shin OH, et al. (2005) Evolutionarily conserved multiple c2 domain proteins with two transmembrane regions (mctps) and unusual ca2+ binding properties. J Biol Chem 280 (2): 1641-1651.Lalani SR, et al. (2013) Mctp2 is a dosage-sensitive gene required for cardiac outflow tract development. Hum Mol Genet 22 (21): 4339-4348.Joshi AS, et al. (2018) Lipid droplet and peroxisome biogenesis occur at the same er subdomains. Nat Commun 9 (1): 2940.Djurovic S, et al. (2009) Association of mctp2 gene variants with schizophrenia in three independent samples of scandinavian origin (scope). Psychiatry Res 168 (3): 256-258.