Western Blot (WB)
(RMI1 Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the RMI1 antibody detected the RMI1 protein (arrow).)
Rabbit anti-Human RMI1 Polyclonal Antibody | anti-RMI1 antibody
RMI1, NT (RMI1, C9orf76, RecQ-mediated genome instability protein 1, BLM-associated protein of 75kD, FAAP75) (FITC)
Gene Names
RMI1; BLAP75; FAAP75; C9orf76; RP11-346I8.1
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
RMI1; Polyclonal Antibody; NT (RMI1; C9orf76; RecQ-mediated genome instability protein 1; BLM-associated protein of 75kD; FAAP75) (FITC); FAAP75; anti-RMI1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-RMI1 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
RMI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-133 amino acids from the N-terminal region of human RMI1.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(RMI1 Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the RMI1 antibody detected the RMI1 protein (arrow).)
Western Blot (WB)
(RMI1 Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the RMI1 antibody detected the RMI1 protein (arrow).)
Related Product Information for anti-RMI1 antibody
RMI1 is a component of protein complexes that limit DNA crossover formation via the dissolution of double Holliday junctions (Raynard et al., 2006 [PubMed 16595695]).[supplied by OMIM].
Product Categories/Family for anti-RMI1 antibody
References
Yang, J., et al. J. Biol. Chem. 285(28):21426-21436(2010). Frank, B., et al. Carcinogenesis 31(3):442-445(2010). Barber, M.J., et al. PLoS ONE 5 (3), E9763 (2010): Luedeke, M., et al. Cancer Epidemiol. Biomarkers Prev. 18(11):3030-3035(2009). Broberg, K., et al. BMC Cancer 9, 140 (2009).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
70,144 Da
NCBI Official Full Name
recQ-mediated genome instability protein 1
NCBI Official Synonym Full Names
RecQ mediated genome instability 1
NCBI Official Symbol
RMI1
NCBI Official Synonym Symbols
BLAP75; FAAP75; C9orf76; RP11-346I8.1
NCBI Protein Information
recQ-mediated genome instability protein 1; BLM-associated protein 75 kDa; BLM-associated polypeptide, 75 kDa; RMI1, RecQ mediated genome instability 1, homolog; homolog of yeast RecQ-mediated genome instability 1 (RMI1)
UniProt Protein Name
RecQ-mediated genome instability protein 1
Protein Family
UniProt Gene Name
RMI1
UniProt Synonym Gene Names
C9orf76; BLAP75
UniProt Entry Name
RMI1_HUMAN
NCBI Description
RMI1 is a component of protein complexes that limit DNA crossover formation via the dissolution of double Holliday junctions (Raynard et al., 2006 [PubMed 16595695]).[supplied by OMIM, Mar 2008]
Uniprot Description
RMI1: Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Promotes TOP3A binding to double Holliday junctions (DHJ) and hence stimulates TOP3A-mediated dissolution. Required for BLM phosphorylation during mitosis. Within the BLM complex, required for BLM and TOP3A stability. Belongs to the RMI1 family.
Protein type: DNA repair, damage
Chromosomal Location of Human Ortholog: 9q21.32
Cellular Component: nucleoplasm; nucleus
Molecular Function: protein binding
Biological Process: multicellular organism growth; response to glucose stimulus; DNA replication; glucose homeostasis; reduction of food intake in response to dietary excess
Research Articles on RMI1
Similar Products
Product Notes
The RMI1 rmi1 (Catalog #AAA6342338) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RMI1, NT (RMI1, C9orf76, RecQ-mediated genome instability protein 1, BLM-associated protein of 75kD, FAAP75) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RMI1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the RMI1 rmi1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RMI1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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