Rabbit anti-Human RLN1/RLN2 Polyclonal Antibody | anti-RLN1/RLN2 antibody

RLN1/RLN2, CT (RLN1, Prorelaxin H1, Relaxin B chain, Relaxin A chain) (AP)

Cat. Num. AAA6342247

• Gene Names: RLN1; H1; H1RLX; RLXH1; bA12D24.3.1; bA12D24.3.2
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RLN1/RLN2; Polyclonal Antibody; CT (RLN1; Prorelaxin H1; Relaxin B chain; Relaxin A chain) (AP); RLN1; Relaxin A chain; anti-RLN1/RLN2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RLN1/RLN2.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-RLN1/RLN2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 136-165 in the C-terminal region of human RLN1/RLN2, UniProt Accession #P04808.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in ZR-75-1 cell line lysates (35ug/lane) usingMBS649479. This demonstrates the RLN1/RLN2 antibody detected the RLN1/RLN2 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue using MBS649479 followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MBS649479 for immunohistochemistry.)

Related Product Information for anti-RLN1/RLN2 antibody

Relaxins are known endocrine and autocrine/paracrine hormones, belonging to the insulin gene superfamily. In the human there are three non-allelic relaxin genes, RLN1, RLN2 and RLN3. RLN1 and RLN2 share high sequence homology. This encoded protein is synthesized as a single-chain polypeptide but the active form consists of an A chain and a B chain linked by disulfide bonds; however, their exact cleavage sites have not been described. Relaxin is produced by the ovary, and targets the mammalian reproductive system to ripen the cervix, elongate the pubic symphysis and inhibit uterine contraction. It may have additional roles in enhancing sperm motility, regulating blood pressure, controlling heart rate and releasing oxytocin and vasopressin. This gene has multiple polyadenylation sites. There are multiple alternatively spliced transcript variants described for this gene but their full length nature is not known yet. [provided by RefSeq].

Product Categories/Family for anti-RLN1/RLN2 antibody

Antibodies; Spermatogenesis; Testis Proteins

References

1. Radestock, Y., et al. Mol. Cancer Res. 8(4):494-506 (2010). 2. Davila, S., et al. Genes Immun. 11(3):232-238 (2010). 3. Ferlin, A., et al. Bone 46(2):504-513 (2010). 4. Vogel, I., et al. In Vivo 23(6):1005-1009 (2009). 5. Anumba, D.O., et al. Eur. J. Obstet. Gynecol. Reprod. Biol. 147(1):41-45 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 5902052
• NCBI GeneID: 6013
• NCBI Accession #: NP_008842.1
• NCBI GenBank Nucleotide #: NM_006911.3
• UniProt Accession #: P04808; P04090
• Molecular Weight: 13,011 Da
• NCBI Official Full Name: prorelaxin H1 preproprotein
• NCBI Official Synonym Full Names: relaxin 1
• NCBI Official Symbol: RLN1
• NCBI Official Synonym Symbols: H1; H1RLX; RLXH1; bA12D24.3.1; bA12D24.3.2
• NCBI Protein Information: prorelaxin H1
• UniProt Protein Name: Prorelaxin H1
• UniProt Gene Name: RLN1
• UniProt Entry Name: REL1_HUMAN

NCBI Description

Relaxins are known endocrine and autocrine/paracrine hormones, belonging to the insulin gene superfamily. In humans there are three non-allelic relaxin genes, RLN1, RLN2 and RLN3, where RLN1 and RLN2 share high sequence homology. The protein encoded by this gene is synthesized as a single-chain polypeptide but the active form consists of an A chain and a B chain linked by disulfide bonds. Relaxin is produced by the ovary, and targets the mammalian reproductive system to ripen the cervix, elongate the pubic symphysis and inhibit uterine contraction. It may have additional roles in enhancing sperm motility, regulating blood pressure, controlling heart rate and releasing oxytocin and vasopressin. [provided by RefSeq, Jan 2013]

Uniprot Description

RLN1: Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals. May be involved in remodeling of connective tissues during pregnancy, promoting growth of pubic ligaments and ripening of the cervix. Belongs to the insulin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9p24.1

Cellular Component: extracellular region

Molecular Function: hormone activity

Biological Process: female pregnancy; signal transduction