Rabbit anti-Mouse RIT1 Polyclonal Antibody | anti-RIT1 antibody

RIT1, NT (RIT1, RIBB, RIT, ROC1, GTP-binding protein Rit1, Ras-like protein expressed in many tissues, Ras-like without CAAX protein 1) (FITC)

Cat. Num. AAA6342206

• Gene Names: RIT1; NS8; RIT; RIBB; ROC1
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RIT1; Polyclonal Antibody; NT (RIT1; RIBB; RIT; ROC1; GTP-binding protein Rit1; Ras-like protein expressed in many tissues; Ras-like without CAAX protein 1) (FITC); Ras-like without CAAX protein 1; anti-RIT1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 219

• Applicable Applications for anti-RIT1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RIT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-70 amino acids from the N-terminal region of human RIT1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RIT1 Antibody (N-term) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the RIT1 antibody detected the RIT1 protein (arrow).)

Related Product Information for anti-RIT1 antibody

RIT belongs to the RAS (HRAS; MIM 190020) subfamily of small GTPases (Hynds et al., 2003 [PubMed 12668729]).[supplied by OMIM].

Product Categories/Family for anti-RIT1 antibody

Antibodies; Binding Proteins

References

Lee, Y.R., et al. EMBO J. 29(10):1748-1761(2010). Katiyar, S., et al. EMBO Rep. 10(8):866-872(2009). Sonuga-Barke, E.J., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 147B (8), 1359-1368 (2008): Hu, J., et al. Genes Dev. 22(7):866-871(2008). Wu, C., et al. Proteomics 7(11):1775-1785(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 5902050
• NCBI GeneID: 6016
• NCBI Accession #: NP_008843.1
• NCBI GenBank Nucleotide #: NP_008843.1
• UniProt Accession #: Q92963
• NCBI Official Full Name: GTP-binding protein Rit1 isoform 2
• NCBI Official Synonym Full Names: Ras like without CAAX 1
• NCBI Official Symbol: RIT1
• NCBI Official Synonym Symbols: NS8; RIT; RIBB; ROC1
• NCBI Protein Information: GTP-binding protein Rit1
• UniProt Protein Name: GTP-binding protein Rit1
• Protein Family: GTP-binding protein
• UniProt Gene Name: RIT1
• UniProt Synonym Gene Names: RIBB; RIT; ROC1
• UniProt Entry Name: RIT1_HUMAN

NCBI Description

This gene encodes a member of a subfamily of Ras-related GTPases. The encoded protein is involved in regulating p38 MAPK-dependent signaling cascades related to cellular stress. This protein also cooperates with nerve growth factor to promote neuronal development and regeneration. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]

Uniprot Description

RIT1: a small G protein of the Ras family. Plays a crucial role in coupling NGF stimulation to the activation of both EPHB2 and p38-alpha signaling pathways and in NGF- dependent neuronal differentiation. Stimulation of the NGF and EGF receptor signaling pathways results in rapid and prolonged activation. Interacts with afadin, the C-terminal domain of RalGDS and RLF, but not with RIN1 and PIK3CA. RLF binds exclusively to the active GTP-bound form. Strongly interacts with BRAF, but only weakly with c-Raf. BRAF and c-RAF association is dependent upon the GTP-bound state. Interacts with RGL3. Shows rapid uncatalyzed guanine nucleotide dissociation rates, which are much faster than those of most Ras subfamily members.

Protein type: G protein; G protein, monomeric; G protein, monomeric, Ras

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: plasma membrane

Molecular Function: calmodulin binding; protein binding; GTP binding

Biological Process: nerve growth factor receptor signaling pathway; small GTPase mediated signal transduction; Ras protein signal transduction; signal transduction

Disease: Noonan Syndrome 8