Western Blot (WB)
(Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane: 293T cells, Primary antibody: 820434(CLEC4D Antibody) at dilution 1/123, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)
Rabbit anti-Human CLEC4D Polyclonal Antibody | anti-CLEC4D antibody
CLEC4D Rabbit pAb
Gene Names
CLEC4D; MCL; MPCL; CLEC6; CLEC-6; CLECSF8
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity purified
Synonyms
CLEC4D; Polyclonal Antibody; CLEC4D Rabbit pAb; C-type lectin domain family 4; member D; MCL; MPCL; CLEC6; CLEC-6; CLECSF8; anti-CLEC4D antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Expressed weakly in peripheral blood leukocytes, bone marrow and spleen. Expression is confined mostly in monocytes and macrophage and seems to be up-regulated by IL-6, IL-10, TNF-alpha and IFN-gamma.
Purity/Purification
Affinity purified
Form/Format
pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Applicable Applications for anti-CLEC4D antibody
ELISA (EIA), Western Blot (WB)
Application Notes
WB: 1:500-1:2000
ELISA: 1:2000-1:5000
ELISA: 1:2000-1:5000
Immunogen
Fusion protein of human CLEC4D
Conjugation
Unconjugated
Molecular Function
A calcium-dependent lectin involved in innate recognition of pathogen-associated molecular patterns (PAMPs). Interacts with signaling adapter Fc receptor gamma chain/FCER1G, likely via CLEC4E, to form a functional complex in myeloid cells. Binding of mycobacterial trehalose 6, 6'-dimycolate (TDM) to this receptor complex leads to phosphorylation of the immunoreceptor tyrosine-based activation motif (ITAM) of FCER1G, triggering activation of SYK, CARD9 and NF-kappa-B, consequently driving maturation of antigen-presenting cells and shaping antigen-specific priming of T-cells toward effector T-helper 1 and T-helper 17 cell subtypes (PubMed:23602766). Functions as an endocytic receptor. May be involved in antigen uptake at the site of infection, either for clearance of the antigen, or for processing and further presentation to T cells (PubMed:14971047).
Modification
Unmodified
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane: 293T cells, Primary antibody: 820434(CLEC4D Antibody) at dilution 1/123, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)
Western Blot (WB)
(Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane: 293T cells, Primary antibody: 820434(CLEC4D Antibody) at dilution 1/123, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)
Related Product Information for anti-CLEC4D antibody
This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region.
Product Categories/Family for anti-CLEC4D antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
215
NCBI Official Full Name
C-type lectin domain family 4 member D
NCBI Official Synonym Full Names
C-type lectin domain family 4, member D
NCBI Official Symbol
CLEC4D
NCBI Official Synonym Symbols
MCL; MPCL; CLEC6; CLEC-6; CLECSF8
NCBI Protein Information
C-type lectin domain family 4 member D; C-type lectin receptor; macrophage C-type lectin; C-type lectin-like receptor 6; C-type lectin superfamily member 8; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 8
UniProt Protein Name
C-type lectin domain family 4 member D
Protein Family
UniProt Gene Name
CLEC4D
UniProt Synonym Gene Names
CLECSF8; MCL; CLEC-6
UniProt Entry Name
CLC4D_HUMAN
NCBI Description
This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. [provided by RefSeq, Jul 2008]
Uniprot Description
CLEC4D: Functions as an endocytic receptor. May be involved in antigen uptake at the site of infection, either for clearance of the antigen, or for processing and further presentation to T cells.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 12p13.31
Cellular Component: integral to membrane
Molecular Function: carbohydrate binding
Biological Process: innate immune response
Research Articles on CLEC4D
Similar Products
Product Notes
The CLEC4D clec4d (Catalog #AAA4757762) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CLEC4D Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CLEC4D can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). WB: 1:500-1:2000 ELISA: 1:2000-1:5000. Researchers should empirically determine the suitability of the CLEC4D clec4d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CLEC4D, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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