Western Blot (WB)
(Anti- RIP antibody, MBS176361, Western blottingAll lanes: Anti RIP (MBS176361) at 0.5ug/mlWB: HELA Whole Cell Lysate at 40ugPredicted bind size: 76KDObserved bind size: 76KD )
Rabbit anti-Human RIP Polyclonal Antibody | anti-RIPK1 antibody
Anti-RIP antibody
Western Blot (WB)
(Anti- RIP antibody, MBS176361, Western blottingAll lanes: Anti RIP (MBS176361) at 0.5ug/mlWB: HELA Whole Cell Lysate at 40ugPredicted bind size: 76KDObserved bind size: 76KD )
Western Blot (WB)
(Anti- RIP antibody, MBS176361, Western blottingAll lanes: Anti RIP (MBS176361) at 0.5ug/mlWB: HELA Whole Cell Lysate at 40ugPredicted bind size: 76KDObserved bind size: 76KD )
Background: RIPK1(Regulator of G Protein Signaling 3), also called RIP, is an enzyme that in humans is encoded by the RIPK1 gene. Members of the TRAF protein family have been implicated in the activation of NF-kappa-B by the TNF superfamily. By yeast 2-hybrid and coimmunoprecipitation studies using mammalian cell extracts, Hsu et al.(1996) showed that RIP interacts with TRADD, TRAF1, TRAF2, and TRAF3. Hartz(2012) mapped the RIPK1 gene to chromosome 6p25.2 based on an alignment of the RIPK1 sequence with the genomic sequence. Stanger et al.(1995) found that overexpression of Rip in mammalian cells induced morphologic changes characteristic of apoptosis. They suggested that RIP may be an important element in the signal transduction machinery that mediates programmed cell death.
NCBI and Uniprot Product Information
Uniprot Description
RIPK1: Serine-threonine kinase which transduces inflammatory and cell-death signals (necroptosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules. Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death. RIPK1 and RIPK3 association, in particular, forms a necroptosis-inducing complex. Interacts (via RIP homotypic interaction motif) with RIPK3 (via RIP homotypic interaction motif); this interaction induces RIPK1 necroptosis-specific phosphorylation, formation of the necroptosis-inducing complex. Interacts (via the death domain) with TNFRSF6 (via the death domain) and TRADD (via the death domain). Is recruited by TRADD to TNFRSF1A in a TNF-dependent process. Binds RNF216, EGFR, IKBKG, TRAF1, TRAF2 and TRAF3. Interacts with BNLF1. Interacts with SQSTM1 upon TNF-alpha stimulation. May interact with MAVS/IPS1. Interacts with ZFAND5. Interacts with RBCK1. Interacts with BIRC2/c-IAP1, BIRC3/c-IAP2 and XIAP/BIRC4. Inhibited by necrostatin-1. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Kinase, protein; EC 2.7.11.1; Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); TKL group; RIPK family
Chromosomal Location of Human Ortholog: 6p25.2
Cellular Component: mitochondrion; cytoplasm; cytosol; receptor complex; lipid raft
Molecular Function: protein serine/threonine kinase activity; identical protein binding; protein binding; ubiquitin protein ligase binding; death receptor binding; protein complex binding; ATP binding; protein kinase activity
Biological Process: apoptosis; positive regulation of apoptosis; protein heterooligomerization; protein amino acid autophosphorylation; positive regulation of JNK cascade; toll-like receptor 3 signaling pathway; activation of JNK activity; activation of NF-kappaB transcription factor; positive regulation of interleukin-8 production; tumor necrosis factor-mediated signaling pathway; positive regulation of interferon type I production; positive regulation of macrophage differentiation; toll-like receptor 4 signaling pathway; protein homooligomerization; caspase activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; MyD88-independent toll-like receptor signaling pathway; negative regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of tumor necrosis factor production; positive regulation of programmed cell death; cellular protein catabolic process; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation
Research Articles on RIPK1
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Product Notes
The RIPK1 ripk1 (Catalog #AAA176361) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RIP antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RIP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the RIPK1 ripk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RIP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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