Rabbit anti-Human TRAP1 Polyclonal Antibody | anti-TRAP1 antibody

TRAP1, CT (TRAP1, HSP75, Heat shock protein 75kD, mitochondrial, TNFR-associated protein 1, Tumor necrosis factor type 1 receptor-associated protein)

Cat. Num. AAA646110

• Gene Names: TRAP1; HSP75; HSP 75; HSP90L; TRAP-1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: TRAP1; Polyclonal Antibody; CT (TRAP1; HSP75; Heat shock protein 75kD; mitochondrial; TNFR-associated protein 1; Tumor necrosis factor type 1 receptor-associated protein); Anti -TRAP1; anti-TRAP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-TRAP1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:10-50
• Immunogen: TRAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 541-570 amino acids from the C-terminal region of human TRAP1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TRAP1 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the TRAP1 antibody detected the TRAP1 protein (arrow).)

Immunohistochemistry (IHC)

(TRAP1 Antibody (C-term) (AP14128b)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TRAP1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-TRAP1 antibody

HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. TRAP1 is a mitochondrial HSP90 protein. Other HSP90 proteins are found in cytosol (see HSP90AA1; MIM 140571) and endoplasmic reticulum (HSP90B1; MIM 191175) (Chen et al., 2005 [PubMed 16269234]).

Product Categories/Family for anti-TRAP1 antibody

Antibodies; Abs to Heat Shock Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 3273383
• NCBI GeneID: 10131
• UniProt Accession #: Q12931; O43642; O75235; Q9UHL5; D3DUC8
• Molecular Weight: 80,110 Da
• NCBI Official Full Name: TRAP1
• NCBI Official Synonym Full Names: TNF receptor-associated protein 1
• NCBI Official Symbol: TRAP1
• NCBI Official Synonym Symbols: HSP75; HSP 75; HSP90L; TRAP-1
• NCBI Protein Information: heat shock protein 75 kDa, mitochondrial; TNFR-associated protein 1; tumor necrosis factor type 1 receptor-associated protein
• UniProt Protein Name: Heat shock protein 75 kDa, mitochondrial
• Protein Family: TNF receptor-associated protein
• UniProt Gene Name: TRAP1
• UniProt Synonym Gene Names: HSP75; HSP 75; TRAP-1
• UniProt Entry Name: TRAP1_HUMAN

NCBI Description

This gene encodes a mitochondrial chaperone protein that is member of the heat shock protein 90 (HSP90) family. The encoded protein has ATPase activity and interacts with tumor necrosis factor type I. This protein may function in regulating cellular stress responses. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]

Uniprot Description

Function: Chaperone that expresses an ATPase activity. Involved in maintaining mitochondrial function and polarization, most likely through stabilization of mitochondrial complex I. Is a negative regulator of mitochondrial respiration able to modulate the balance between oxidative phosphorylation and aerobic glycolysis. The impact of TRAP1 on mitochondrial respiration is probably mediated by modulation of mitochondrial SRC and inhibition of SDHA. Ref.12 Ref.13 Ref.14

Subunit structure: Binds to the intracellular domain of tumor necrosis factor type 1 receptor. Binds to RB1. Interacts with SRC. Interacts with SDHA. Ref.12 Ref.14

Subcellular location: Mitochondrion. Mitochondrion inner membrane. Mitochondrion matrix Ref.14.

Tissue specificity: Found in skeletal muscle, liver, heart, brain, kidney, pancreas, lung, placenta and bladder. Expression is higly reduced in bladder cancer and renal cell carcinoma specimens compared to healthy tissues, but it is increased in other type of tumors. Ref.14

Sequence similarities: Belongs to the heat shock protein 90 family.

Sequence caution: The sequence AAA87704.1 differs from that shown. Reason: Frameshift at position 656.