Western Blot (WB)
(Western Blot analysis of RHOT2 expression in transfected 293T cell line by RHOT2 polyclonal antibody. Lane 1: RHOT2 transfected lysate (67.98kD). Lane 2: Non-transfected lysate.)
Mouse anti-Human RHOT2 Polyclonal Antibody | anti-RHOT2 antibody
RHOT2 (Ras Homolog Gene Family Member T2, ARHT2, C16orf39, Mitochondrial Rho GTPase 2, MIRO2, MIRO-2, hMiro-2, RASL)
Gene Names
RHOT2; MIRO2; MIRO-2
Reactivity
Human
Applications
Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
RHOT2; Polyclonal Antibody; RHOT2 (Ras Homolog Gene Family Member T2; ARHT2; C16orf39; Mitochondrial Rho GTPase 2; MIRO2; MIRO-2; hMiro-2; RASL); Anti -RHOT2 (Ras Homolog Gene Family Member T2; anti-RHOT2 antibody
Host
Mouse
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human RHOT2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Sequence
MRRDVRILLLGEAQVGKTSLILSLVGEEFPEEVPPRAEEITIPADVTPEKVPTHIVDYSEAEQTDEELREEIHKANVVCVVYDVSEEATIEKIRTKWIPLVNGGTTQGPRVPIILVGNKSDLRSGSSMEAVLPIMSQFPEIETCVECSAKNLRNISELFYYAQKAVLHPTAPLYDPEAKQLRPACAQALTRIFRLSDQDLDQALSDEELNAFQKSCFGHPLAPQALEDVKTVVCRNVAGGVREDRLTLDGFLFLNTLFIQRGRHETTWTILRRFGYSDALELTADYLSPLIHVPPGCSTELNHLGYQFVQRVFEKHDQDRDGALSPVELQSLFSVFPAAPWGPELPRTVRTEAGRLPLHGYLCQWTLVTYLDVRSCLGHLGYLGYPTLCEQDQAHAITVTREKRLDQEKGQTQRSVLLCKVVGARGVGKSAFLQAFLGRGLGHQDTREQPPGYAIDTVQVNGQEKYLILCEVGTDGLLATSLDATCDVACLMFDGSDPKSFAHCASVYKHHYMDGQTPCLFVSSKADLPEGVAVSGPSPAEFCRKHRLPAPVPFSCAGPAEPSTTIFTQLATMAAFPHLVHAELHPSSFWLRGLLGVVGAAVAAVLSFSLYRVLVKSQ
Applicable Applications for anti-RHOT2 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Immunogen
Full length human RHOT2, aa1-618 (NP_620124.1).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of RHOT2 expression in transfected 293T cell line by RHOT2 polyclonal antibody. Lane 1: RHOT2 transfected lysate (67.98kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of RHOT2 expression in transfected 293T cell line by RHOT2 polyclonal antibody. Lane 1: RHOT2 transfected lysate (67.98kD). Lane 2: Non-transfected lysate.)
Product Categories/Family for anti-RHOT2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
68,152 Da
NCBI Official Full Name
RHOT2 protein
NCBI Official Symbol
RHOT2
NCBI Official Synonym Symbols
MIRO2; MIRO-2
NCBI Protein Information
mitochondrial Rho GTPase 2; miro protein; mitochondrial Rho 2; ras homolog gene family member T2; ras homolog gene family, member T2
UniProt Protein Name
Mitochondrial Rho GTPase 2
UniProt Gene Name
RHOT2
UniProt Synonym Gene Names
ARHT2; MIRO-2
UniProt Entry Name
MIRO2_BOVIN
Uniprot Description
Function: Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution
By similarity.
Subunit structure: Interacts with the kinesin-binding proteins TRAK1/OIP106 and TRAK2/GRIF1, forming a link between mitochondria and the trafficking apparatus of the microtubules
By similarity.
Subcellular location: Mitochondrion outer membrane; Single-pass type IV membrane protein
By similarity. Note: Colocalizes with MGARP and RHOT2 at the mitochondria
By similarity.
Sequence similarities: Belongs to the mitochondrial Rho GTPase family.Contains 2 EF-hand domains.Contains 2 Miro domains.
Similar Products
Product Notes
The RHOT2 rhot2 (Catalog #AAA6009060) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RHOT2 (Ras Homolog Gene Family Member T2, ARHT2, C16orf39, Mitochondrial Rho GTPase 2, MIRO2, MIRO-2, hMiro-2, RASL) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RHOT2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Researchers should empirically determine the suitability of the RHOT2 rhot2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MRRDVRILLL GEAQVGKTSL ILSLVGEEFP EEVPPRAEEI TIPADVTPEK VPTHIVDYSE AEQTDEELRE EIHKANVVCV VYDVSEEATI EKIRTKWIPL VNGGTTQGPR VPIILVGNKS DLRSGSSMEA VLPIMSQFPE IETCVECSAK NLRNISELFY YAQKAVLHPT APLYDPEAKQ LRPACAQALT RIFRLSDQDL DQALSDEELN AFQKSCFGHP LAPQALEDVK TVVCRNVAGG VREDRLTLDG FLFLNTLFIQ RGRHETTWTI LRRFGYSDAL ELTADYLSPL IHVPPGCSTE LNHLGYQFVQ RVFEKHDQDR DGALSPVELQ SLFSVFPAAP WGPELPRTVR TEAGRLPLHG YLCQWTLVTY LDVRSCLGHL GYLGYPTLCE QDQAHAITVT REKRLDQEKG QTQRSVLLCK VVGARGVGKS AFLQAFLGRG LGHQDTREQP PGYAIDTVQV NGQEKYLILC EVGTDGLLAT SLDATCDVAC LMFDGSDPKS FAHCASVYKH HYMDGQTPCL FVSSKADLPE GVAVSGPSPA EFCRKHRLPA PVPFSCAGPA EPSTTIFTQL ATMAAFPHLV HAELHPSSFW LRGLLGVVGA AVAAVLSFSL YRVLVKSQ. It is sometimes possible for the material contained within the vial of "RHOT2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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