Western Blot (WB)
(RHOT1 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the RHOT1 antibody detected the RHOT1 protein (arrow).)
Rabbit anti-Human RHOT1 Polyclonal Antibody | anti-RHOT1 antibody
RHOT1, NT (RHOT1, ARHT1, Mitochondrial Rho GTPase 1, Rac-GTP-binding protein-like protein, Ras homolog gene family member T1) (AP)
Gene Names
RHOT1; ARHT1; MIRO1; MIRO-1
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
RHOT1; Polyclonal Antibody; NT (RHOT1; ARHT1; Mitochondrial Rho GTPase 1; Rac-GTP-binding protein-like protein; Ras homolog gene family member T1) (AP); mitochondrial Rho GTPase 1; Ras homolog gene family member T1; anti-RHOT1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-RHOT1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
RHOT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 83-110 amino acids from the N-terminal region of human RHOT1.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(RHOT1 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the RHOT1 antibody detected the RHOT1 protein (arrow).)
Western Blot (WB)
(RHOT1 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the RHOT1 antibody detected the RHOT1 protein (arrow).)
Related Product Information for anti-RHOT1 antibody
Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution.
Product Categories/Family for anti-RHOT1 antibody
References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): MacAskill, A.F., et al. Mol. Cell. Neurosci. 40(3):301-312(2009). Wang, X., et al. Cell 136(1):163-174(2009). Saotome, M., et al. Proc. Natl. Acad. Sci. U.S.A. 105(52):20728-20733(2008). Fransson, S., et al. Biochem. Biophys. Res. Commun. 344(2):500-510(2006).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
70,784 Da
NCBI Official Full Name
mitochondrial Rho GTPase 1 isoform 2
NCBI Official Synonym Full Names
ras homolog family member T1
NCBI Official Symbol
RHOT1
NCBI Official Synonym Symbols
ARHT1; MIRO1; MIRO-1
NCBI Protein Information
mitochondrial Rho GTPase 1; mitochondrial Rho (MIRO) GTPase 1; ras homolog gene family, member T1; rac-GTP binding protein-like protein
UniProt Protein Name
Mitochondrial Rho GTPase 1
UniProt Gene Name
RHOT1
UniProt Synonym Gene Names
ARHT1; MIRO-1; hMiro-1
UniProt Entry Name
MIRO1_HUMAN
Uniprot Description
RHOT1: Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution. Belongs to the mitochondrial Rho GTPase family. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: G protein, monomeric; Membrane protein, integral; Hydrolase; Mitochondrial; G protein, monomeric, Rho; EC 3.6.5.-; G protein
Chromosomal Location of Human Ortholog: 17q11.2
Cellular Component: membrane; plasma membrane; cytosol; integral to mitochondrial outer membrane
Molecular Function: GTPase activity; protein binding; GTP binding; calcium ion binding
Biological Process: regulation of small GTPase mediated signal transduction; metabolic process; small GTPase mediated signal transduction; cellular homeostasis; mitochondrion transport along microtubule
Similar Products
Product Notes
The RHOT1 rhot1 (Catalog #AAA6341994) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RHOT1, NT (RHOT1, ARHT1, Mitochondrial Rho GTPase 1, Rac-GTP-binding protein-like protein, Ras homolog gene family member T1) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RHOT1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the RHOT1 rhot1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RHOT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
