Rabbit anti-Human RGS9 Polyclonal Antibody | anti-RGS9 antibody

RGS9, NT (RGS9, Regulator of G-protein signaling 9) (Azide free) (HRP)

Cat. Num. AAA6341822

• Gene Names: RGS9; PERRS; RGS9L
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RGS9; Polyclonal Antibody; NT (RGS9; Regulator of G-protein signaling 9) (Azide free) (HRP); Regulator of G-protein signaling 9; anti-RGS9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-RGS9 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: RGS9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 148-178 amino acids from the N-terminal region of human RGS9.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RGS9 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the RGS9 antibody detected the RGS9 protein (arrow).)

Immunohistochemistry (IHC)

(RGS9 Antibody (N-term) (AP14236a)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RGS9 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-RGS9 antibody

This gene encodes a member of the RGS family of GTPase activating proteins that function in various signaling pathways by accelerating the deactivation of G proteins. This protein is anchored to photoreceptor membranes in retinal cells and deactivates G proteins in the rod and cone phototransduction cascades. Mutations in this gene result in bradyopsia. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-RGS9 antibody

Antibodies; G Proteins

References

Wang, J., et al. Carcinogenesis 31(10):1755-1761(2010). Celver, J., et al. J. Neurochem. 114(3):739-749(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Greenbaum, L., et al. Psychiatr. Genet. 20(1):47-48(2010). Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 126366080
• NCBI GeneID: 8787
• NCBI Accession #: NP_001075424.1; NP_003826.2
• NCBI GenBank Nucleotide #: NM_001081955.2
• UniProt Accession #: O75916; O75573; Q696R2; Q8TD64; Q8TD65; Q9HC32; Q9HC33; A8K3C0
• Molecular Weight: 76,966 Da
• NCBI Official Full Name: regulator of G-protein signaling 9 isoform 2
• NCBI Official Synonym Full Names: regulator of G-protein signaling 9
• NCBI Official Symbol: RGS9
• NCBI Official Synonym Symbols: PERRS; RGS9L
• NCBI Protein Information: regulator of G-protein signaling 9; regulator of G-protein signalling 9
• UniProt Protein Name: Regulator of G-protein signaling 9
• Protein Family: Regulator of G-protein signaling
• UniProt Gene Name: RGS9
• UniProt Synonym Gene Names: RGS9
• UniProt Entry Name: RGS9_HUMAN

Uniprot Description

RGS9: belongs to the 'regulator of G protein signaling' family. Inhibits signal transduction by increasing the GTPAse activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G(t)-alpha. Involved in phototransduction; key element in the recovery phase of visual transduction. Four alternatively spliced isoforms have been described.

Protein type: GAPs, RGS; GAPs

Chromosomal Location of Human Ortholog: 17q24

Cellular Component: cytoplasm; plasma membrane; heterotrimeric G-protein complex; nucleus

Molecular Function: signal transducer activity; protein complex binding; GTPase activator activity

Biological Process: rhodopsin mediated signaling; phototransduction, visible light; nervous system development; regulation of rhodopsin mediated signaling; visual perception; response to estrogen stimulus; dopamine receptor signaling pathway; regulation of G-protein coupled receptor protein signaling pathway; positive regulation of GTPase activity

Disease: Prolonged Electroretinal Response Suppression