Chicken RGS4 ELISA Kit | RGS4 elisa kit

Chicken RGS4 (Regulator of G Protein Signaling 4) ELISA Kit

Cat. Num. AAA2503305

• Gene Names: RGS4; RGP4; SCZD9
• Reactivity: Chicken
• Synonyms: RGS4; Chicken RGS4 (Regulator of G Protein Signaling 4) ELISA Kit; RGS4 elisa kit

• Reactivity: Chicken
• Specificity: This kit recognizes natural and recombinant Chicken RGS4. No significant cross-reactivity or interference between Chicken RGS4 and analogues was observed.
• Sequence Length: 205

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 1.563-100ng/mL
• Sensitivity: Min: 0.938ng/mL; Max: 100ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RGS4 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Chicken RGS4 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to RGS4. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for RGS4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain RGS4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of RGS4. You can calculate the concentration of RGS4 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 5032039
• NCBI GeneID: 5999
• NCBI Accession #: NP_005604.1
• NCBI GenBank Nucleotide #: NM_005613.5
• UniProt Accession #: P49798; A7XA56; A7XA58; A7XA59; A7YVV7; B1APZ3
• Molecular Weight: 21,452 Da
• NCBI Official Full Name: regulator of G-protein signaling 4 isoform 2
• NCBI Official Synonym Full Names: regulator of G-protein signaling 4
• NCBI Official Symbol: RGS4
• NCBI Official Synonym Symbols: RGP4; SCZD9
• NCBI Protein Information: regulator of G-protein signaling 4; schizophrenia disorder 9
• UniProt Protein Name: Regulator of G-protein signaling 4
• Protein Family: Regulator of G-protein signaling
• UniProt Gene Name: RGS4
• UniProt Synonym Gene Names: RGP4; RGS4
• UniProt Entry Name: RGS4_HUMAN

NCBI Description

Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP-bound forms. Regulator of G protein signaling 4 belongs to this family. All RGS proteins share a conserved 120-amino acid sequence termed the RGS domain. Regulator of G protein signaling 4 protein is 37% identical to RGS1 and 97% identical to rat Rgs4. This protein negatively regulate signaling upstream or at the level of the heterotrimeric G protein and is localized in the cytoplasm. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

RGS4: Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Activity on G(z)-alpha is inhibited by phosphorylation of the G-protein. Activity on G(z)-alpha and G(i)-alpha-1 is inhibited by palmitoylation of the G-protein. Expressed in brain and heart. Expressed in brain at protein level. Expressed in prefontal and visual cortex. Isoform 4 and isoform 5 are expressed ubiquitously. Isoform 1, isoform 2 and isoform 3 are not expressed in the cerebellum. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: GAPs, RGS; GAPs

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: protein complex; cytoplasm; plasma membrane; cytosol

Molecular Function: calmodulin binding; G-protein alpha-subunit binding; GTPase activator activity

Biological Process: regulation of G-protein coupled receptor protein signaling pathway; inactivation of MAPK activity; positive regulation of GTPase activity

Disease: Schizophrenia 9