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Western Blot (WB) (All lanes : Anti-RGMB Antibody at 1:2000 dilutionLane 1: U-251 MG whole cell lysateLane 2: 293 whole cell lysateLane 3: Human brain lysateLane 4: Mouse brain lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Rabbit anti-Human, Mouse RGMB Polyclonal Antibody | anti-RGMB antibody

RGMB Antibody

Gene Names
RGMB; DRAGON
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Synonyms
RGMB; Polyclonal Antibody; RGMB Antibody; RGM domain family member B; DRG11-responsive axonal guidance and outgrowth of neurite; DRAGON; anti-RGMB antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Ig
Specificity
This RGMB antibody is generated from a rabbit immunized with a recombinant protein of human RGMB.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
478
Applicable Applications for anti-RGMB antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:2000
Antigen Type
Recombinant Protein
Antigen Source
Human
Cellular Location
Cell membrane; Lipid-anchor, GPI-anchor. Membrane raft
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-RGMB Antibody at 1:2000 dilutionLane 1: U-251 MG whole cell lysateLane 2: 293 whole cell lysateLane 3: Human brain lysateLane 4: Mouse brain lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (All lanes : Anti-RGMB Antibody at 1:2000 dilutionLane 1: U-251 MG whole cell lysateLane 2: 293 whole cell lysateLane 3: Human brain lysateLane 4: Mouse brain lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(All lanes : Anti-RGMB Antibody at 1:2000 dilutionLane 1: 293 whole cell lysateLane 2: Human brain lysateLane 3: Mouse brain lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (All lanes : Anti-RGMB Antibody at 1:2000 dilutionLane 1: 293 whole cell lysateLane 2: Human brain lysateLane 3: Mouse brain lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-RGMB antibody
Member of the repulsive guidance molecule (RGM) family that contributes to the patterning of the developing nervous system (By similarity). Acts as a bone morphogenetic protein (BMP) coreceptor that potentiates BMP signaling (By similarity). Promotes neuronal adhesion (By similarity). May inhibit neurite outgrowth.
Product Categories/Family for anti-RGMB antibody
References
Ota T.,et al.Nat. Genet. 36:40-45 (2004). Schmutz J.,et al.Nature 431:268-274 (2004). Liu X.,et al.Biochem. Biophys. Res. Commun. 382:795-800 (2009). Bell C.H.,et al.Science 341:77-80 (2013).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
47547 Da
NCBI Official Full Name
RGM domain family member B
NCBI Official Synonym Full Names
repulsive guidance molecule family member b
NCBI Official Symbol
RGMB
NCBI Official Synonym Symbols
DRAGON
NCBI Protein Information
RGM domain family member B
UniProt Protein Name
RGM domain family member B
Protein Family
UniProt Gene Name
RGMB
UniProt Synonym Gene Names
DRAGON

NCBI Description

RGMB is a glycosylphosphatidylinositol (GPI)-anchored member of the repulsive guidance molecule family (see RGMA, MIM 607362) and contributes to the patterning of the developing nervous system (Samad et al., 2005 [PubMed 15671031]).[supplied by OMIM, Apr 2009]

Uniprot Description

RGMB: Member of the repulsive guidance molecule (RGM) family that contributes to the patterning of the developing nervous system. Acts as a bone morphogenetic protein (BMP) coreceptor that potentiates BMP signaling. Promotes neuronal adhesion. May inhibit neurite outgrowth. Belongs to the repulsive guidance molecule (RGM) family.

Protein type: Cell adhesion; Membrane protein, GPI anchor; Transcription regulation

Chromosomal Location of Human Ortholog: 5q15

Cellular Component: anchored to plasma membrane; ER-Golgi intermediate compartment; plasma membrane

Molecular Function: coreceptor activity; identical protein binding

Biological Process: BMP signaling pathway; cell adhesion; positive regulation of transcription, DNA-dependent; signal transduction

Research Articles on RGMB

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Product Notes

The RGMB rgmb (Catalog #AAA9231098) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RGMB Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RGMB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:2000. Researchers should empirically determine the suitability of the RGMB rgmb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RGMB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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