Goat RFX5 Polyclonal Antibody | anti-RFX5 antibody

Goat anti-RFX5 Antibody

Cat. Num. AAA422899

• Reactivity: Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Pig, Dog, Cow, Rabbit
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: RFX5; Polyclonal Antibody; Goat anti-RFX5 Antibody; regulatory factor X; 5 (influences HLA class II expression); OTTHUMP00000196318; 5; OTTHUMP00000196318 antibody; 5 antibody; 5 (influences HLA class II expression) antibody; RFX5 antibody; anti-RFX5 antibody

• Host: Goat
• Reactivity: Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Pig, Dog, Cow, Rabbit
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: TGDKSSEPSTLSNE
• Sequence Length: 616

• Applicable Applications for anti-RFX5 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 1000.; Western Blot: Approx 70kDa band observed in lysates of cell line Daudi and in NIH3T3 nuclear lysates (calculated MW of 65.3kDa according to NP_000440.1). Recommended concentration: 1-3ug/ml.
• Immunogen: Peptide with sequence C-TGDKSSEPSTLSNE, from the internal region of the protein sequence according to NP_000440.1.
• Epitope: Internal region
• Note: Reported variants represent identical protein: NP_000440.1, NP_001020774.1
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((2ug/ml) staining of Daudi lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB)

((2ug/ml) staining of NIH3T3 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 4557843
• NCBI GeneID: 5993
• NCBI Accession #: NP_000440.1
• NCBI GenBank Nucleotide #: NM_000449.3
• Molecular Weight: 60,513 Da
• NCBI Official Full Name: DNA-binding protein RFX5
• NCBI Official Synonym Full Names: regulatory factor X5
• NCBI Official Symbol: RFX5
• NCBI Protein Information: DNA-binding protein RFX5
• UniProt Protein Name: DNA-binding protein RFX5
• Protein Family: DNA-binding protein
• UniProt Gene Name: RFX5
• UniProt Entry Name: RFX5_HUMAN

NCBI Description

A lack of MHC-II expression results in a severe immunodeficiency syndrome called MHC-II deficiency, or the bare lymphocyte syndrome (BLS; MIM 209920). At least 4 complementation groups have been identified in B-cell lines established from patients with BLS. The molecular defects in complementation groups B, C, and D all lead to a deficiency in RFX, a nuclear protein complex that binds to the X box of MHC-II promoters. The lack of RFX binding activity in complementation group C results from mutations in the RFX5 gene encoding the 75-kD subunit of RFX (Steimle et al., 1995). RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. Multiple alternatively spliced transcript variants have been found but the full-length natures of only two have been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

RFX5: Activates transcription from class II MHC promoters. Recognizes X-boxes. Mediates cooperative binding between RFX and NF-Y. RFX binds the X1 box of MHC-II promoters. Defects in RFX5 are a cause of bare lymphocyte syndrome type 2 (BLS2); also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency. BLS2 is a severe combined immunodeficiency disease with early onset. It is characterized by a profound defect in constitutive and interferon-gamma induced MHC II expression, absence of cellular and humoral T-cell response to antigen challenge, hypogammaglobulinemia and impaired antibody production. The consequence include extreme susceptibility to viral, bacterial and fungal infections. Belongs to the RFX family.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: nucleus

Molecular Function: DNA binding; protein binding; transcription factor activity

Biological Process: negative regulation of transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent

Disease: Bare Lymphocyte Syndrome, Type Ii