Rabbit anti-Human RFX5 Polyclonal Antibody | anti-RFX5 antibody

RFX5 Antibody (Center)

Cat. Num. AAA9208968

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: RFX5; Polyclonal Antibody; RFX5 Antibody (Center); DNA-binding protein RFX5; Regulatory factor X 5; anti-RFX5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This RFX5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 237-266 amino acids from the Central region of human RFX5.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 237-266
• Sequence Length: 616

• Applicable Applications for anti-RFX5 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(RFX5 Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the RFX5 antibody detected the RFX5 protein (arrow).)

Related Product Information for anti-RFX5 antibody

A lack of MHC-II expression results in a severe
immunodeficiency syndrome called MHC-II deficiency, or the bare
lymphocyte syndrome (BLS; MIM 209920). At least 4 complementation
groups have been identified in B-cell lines established from
patients with BLS. The molecular defects in complementation groups
B, C, and D all lead to a deficiency in RFX, a nuclear protein
complex that binds to the X box of MHC-II promoters. The lack of
RFX binding activity in complementation group C results from
mutations in the RFX5 gene encoding the 75-kD subunit of RFX
(Steimle et al., 1995). RFX5 is the fifth member of the growing
family of DNA-binding proteins sharing a novel and highly
characteristic DNA-binding domain called the RFX motif. Multiple
alternatively spliced transcript variants have been found but the
full-length natures of only two have been determined. [provided by
RefSeq].

Product Categories/Family for anti-RFX5 antibody

Immunology

References

Laird, K.M., et al. J. Mol. Biol. 403(1):40-51(2010)
Kong, X., et al. J. Mol. Cell. Cardiol. 46(3):292-299(2009)
Garvie, C.W., et al. Biochim. Biophys. Acta 1779(12):797-804(2008)
Ennis, S., et al. Lancet 372(9652):1828-1834(2008)
Xu, Y., et al. J. Biol. Chem. 282(36):26046-26056(2007)

NCBI and Uniprot Product Information

• NCBI GI #: 4557843
• NCBI GeneID: 5993
• NCBI Accession #: NP_000440.1; NP_001020774.1
• NCBI GenBank Nucleotide #: NM_000449.3
• UniProt Accession #: P48382; Q5VWC3; B7Z848; D3DV19; E9PFU4
• Molecular Weight: 65323
• NCBI Official Full Name: DNA-binding protein RFX5
• NCBI Official Synonym Full Names: regulatory factor X, 5 (influences HLA class II expression)
• NCBI Official Symbol: RFX5
• NCBI Protein Information: DNA-binding protein RFX5
• UniProt Protein Name: DNA-binding protein RFX5
• Protein Family: DNA-binding protein
• UniProt Gene Name: RFX5
• UniProt Entry Name: RFX5_HUMAN

NCBI Description

A lack of MHC-II expression results in a severe immunodeficiency syndrome called MHC-II deficiency, or the bare lymphocyte syndrome (BLS; MIM 209920). At least 4 complementation groups have been identified in B-cell lines established from patients with BLS. The molecular defects in complementation groups B, C, and D all lead to a deficiency in RFX, a nuclear protein complex that binds to the X box of MHC-II promoters. The lack of RFX binding activity in complementation group C results from mutations in the RFX5 gene encoding the 75-kD subunit of RFX (Steimle et al., 1995). RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. Multiple alternatively spliced transcript variants have been found but the full-length natures of only two have been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

RFX5: Activates transcription from class II MHC promoters. Recognizes X-boxes. Mediates cooperative binding between RFX and NF-Y. RFX binds the X1 box of MHC-II promoters. Defects in RFX5 are a cause of bare lymphocyte syndrome type 2 (BLS2); also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency. BLS2 is a severe combined immunodeficiency disease with early onset. It is characterized by a profound defect in constitutive and interferon-gamma induced MHC II expression, absence of cellular and humoral T-cell response to antigen challenge, hypogammaglobulinemia and impaired antibody production. The consequence include extreme susceptibility to viral, bacterial and fungal infections. Belongs to the RFX family.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: nucleus

Molecular Function: protein binding; DNA binding; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter

Disease: Bare Lymphocyte Syndrome, Type Ii