Rabbit anti-Human RFK Polyclonal Antibody | anti-RFK antibody

RFK, NT (Riboflavin Kinase, ATP:riboflavin 5'-phosphotransferase, Flavokinase) (MaxLight 405)

Cat. Num. AAA6497580

• Gene Names: RFK; RIFK
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: RFK; Polyclonal Antibody; NT (Riboflavin Kinase; ATP:riboflavin 5'-phosphotransferase; Flavokinase) (MaxLight 405); anti-RFK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RFK.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-RFK antibody: Immunohistochemistry (IHC), FLISA
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human RFK (KLH).
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Product Categories/Family for anti-RFK antibody

Antibodies; Protein Kinases

References

1. Karthikeyan, S., et al., Biochemistry 42(43):12532-12538 (2003). 2. Karthikeyan, S., et al., Structure (Camb.) 11(3):265-273 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 269973877
• NCBI GeneID: 55312
• NCBI Accession #: NP_060809
• NCBI GenBank Nucleotide #: NM_018339.5
• UniProt Accession #: Q969G6; Q5JSG9; Q9NUT7
• Molecular Weight: 20.5 kDa (182 aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: riboflavin kinase
• NCBI Official Synonym Full Names: riboflavin kinase
• NCBI Official Symbol: RFK
• NCBI Official Synonym Symbols: RIFK
• NCBI Protein Information: riboflavin kinase
• UniProt Protein Name: Riboflavin kinase
• UniProt Gene Name: RFK
• UniProt Entry Name: RIFK_HUMAN

NCBI Description

Riboflavin kinase (RFK; EC 2.7.1.26) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis (Karthikeyan et al., 2003 [PubMed 12623014]).[supplied by OMIM, Nov 2009]

Uniprot Description

RFK: Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN).

Protein type: Cofactor and Vitamin Metabolism - riboflavin; EC 2.7.1.26; Kinase, other

Chromosomal Location of Human Ortholog: 9q21.13

Cellular Component: mitochondrion; cytoplasm; cytosol

Molecular Function: riboflavin kinase activity; metal ion binding; ATP binding

Biological Process: riboflavin metabolic process; vitamin metabolic process; apoptosis; FMN biosynthetic process; riboflavin biosynthetic process; phosphorylation; water-soluble vitamin metabolic process; positive regulation of NAD(P)H oxidase activity