SDS-Page
(Recombinant IDH1 (R132C) protein gel 10% SDS-PAGE Coomassie staining MW: 47.7 kDa Purity: > 90% )
IDH1 recombinant protein
Recombinant IDH1 (R132C) protein
Synonyms
IDH1; Recombinant IDH1 (R132C) protein; Isocitrate Dehydrogenase (NADP(+)) 1; Cytosolic; Oxalosuccinate Decarboxylase; NADP(+)-Specific ICDH; IDH1 recombinant protein
Host
E Coli
Form/Format
Recombinant IDH1 (R132C) protein is supplied at a concentration of 0.8ug/ul in 25mM Tris pH8.0, 300mM NaCl, 5% glycerol.
Protein Species
Human
Tag
His-Tag
Notes
This protein is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Activity Assay Conditions: 10uM NADPH and 1uM a-KG were incubated with 100nm IDH1 (R132C) protein in 200ul reaction system containing 50mM Tris-HCl pH 7.4, 150mM NaCl, 10mM MgCl2 and 0.03% BSA. All the operations and reactions were performed at room temperature. Depletion of NADPH was monitored continuously at Abs 340nm for 20 min.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant IDH1 (R132C) protein gel 10% SDS-PAGE Coomassie staining MW: 47.7 kDa Purity: > 90% )
SDS-Page
(Recombinant IDH1 (R132C) protein gel 10% SDS-PAGE Coomassie staining MW: 47.7 kDa Purity: > 90% )
Testing Data
(Recombinant IDH1 (R131C) protein activity assay 10 uM NADPH and 1 uM a-KG were incubated with 100 nM IDH1 (R132C) protein in 200 ul reaction system containing 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 10 mM MgCl2 and 0.03% BSA (room temperature). Depletion of NADPH was monitored continuously at Abs 340 nm for 20 min.)
Testing Data
(Recombinant IDH1 (R131C) protein activity assay 10 uM NADPH and 1 uM a-KG were incubated with 100 nM IDH1 (R132C) protein in 200 ul reaction system containing 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 10 mM MgCl2 and 0.03% BSA (room temperature). Depletion of NADPH was monitored continuously at Abs 340 nm for 20 min.)
Related Product Information for IDH1 recombinant protein
Short Description: Recombinant IDH1 (R132C) protein was expressed in E Coli as the full-length protein (accession number AAH93020.1) with a point mutation Arg132Cys and a C-terminal 6xHis-tag. The molecular weight of the protein is 47.7 kDa. It is suitable for peroxisome and NADPH regulation assays, as well as for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Background: IDH1 (Isocitrate Dehydrogenase (NADP(+)) 1, Cytosolic, also known as HEL-216, HEL-S-26, IDCD, IDH, IDP, IDPC, PICD) is a member of isocitrate dehydrogenases, which catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. IDH1 is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Mutations in human cytosolic isocitrate dehydrogenase I (IDH1) occur somatically in >70% of grade II-III gliomas and secondary glioblastomas, and in 8.5% of acute myeloid leukemias (AML). Mutations have also been reported in cancers of the colon and prostate. To date, mutations in at least four active site arginine residues IDH1 R100, IDH1 R132, IDH2 R140, and IDH2 R172 have been shown to result in the neomorphic production of R(-)-2-hydroxyglutarate (2HG), although these mutants lack the wild-type enzyme's ability to convert isocitrate to a-ketoglutarate (a-KG, 2OG). Among of them, IDH1 R100A is affected in adult glioma.
Background: IDH1 (Isocitrate Dehydrogenase (NADP(+)) 1, Cytosolic, also known as HEL-216, HEL-S-26, IDCD, IDH, IDP, IDPC, PICD) is a member of isocitrate dehydrogenases, which catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. IDH1 is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Mutations in human cytosolic isocitrate dehydrogenase I (IDH1) occur somatically in >70% of grade II-III gliomas and secondary glioblastomas, and in 8.5% of acute myeloid leukemias (AML). Mutations have also been reported in cancers of the colon and prostate. To date, mutations in at least four active site arginine residues IDH1 R100, IDH1 R132, IDH2 R140, and IDH2 R172 have been shown to result in the neomorphic production of R(-)-2-hydroxyglutarate (2HG), although these mutants lack the wild-type enzyme's ability to convert isocitrate to a-ketoglutarate (a-KG, 2OG). Among of them, IDH1 R100A is affected in adult glioma.
Product Categories/Family for IDH1 recombinant protein
NCBI and Uniprot Product Information
Molecular Weight
Molecular weight of the protein is 47.7kDa
Protein Family
Similar Products
Product Notes
The IDH1 (Catalog #AAA388310) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "IDH1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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