Rabbit anti-Human RFFL Polyclonal Antibody | anti-RFFL antibody

RFFL, CT (RFFL, RNF189, RNF34L, E3 ubiquitin-protein ligase rififylin, Caspase regulator CARP2, Caspases-8 and-10-associated RING finger protein 2, FYVE-RING finger protein Sakura, RING finger and FYVE-like domain-containing protein 1, RING finger protein

Cat. Num. AAA6341480

• Gene Names: RFFL; CARP2; FRING; CARP-2; RNF189; RNF34L; RIFIFYLIN
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RFFL; Polyclonal Antibody; CT (RFFL; RNF189; RNF34L; E3 ubiquitin-protein ligase rififylin; Caspase regulator CARP2; Caspases-8 and-10-associated RING finger protein 2; FYVE-RING finger protein Sakura; RING finger and FYVE-like domain-containing protein 1; RING finger protein; RING finger protein 189; RING finger protein 34-like; anti-RFFL antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-RFFL antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RFFL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 265-294 amino acids from the C-terminal region of human RFFL.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RFFL Antibody (C-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the RFFL antibody detected the RFFL protein (arrow).)

Related Product Information for anti-RFFL antibody

RFFL has E3 ubiquitin protein ligase activity. Regulates the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. Has anti-apoptotic activity. May bind phosphatidylinositol phosphates.

Product Categories/Family for anti-RFFL antibody

Antibodies; Ubiquitin Ligases

References

Shimada, M., et al. Hum. Genet. 128(4):433-441(2010). Hosgood, H.D. III, et al. Occup Environ Med 66(12):848-853(2009). Newton-Cheh, C., et al. Nat. Genet. 41(4):399-406(2009). Liao, W., et al. Curr. Biol. 18(9):641-649(2008). Yang, W., et al. Cell Cycle 7(5):670-682(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 17432433
• NCBI GeneID: 117584
• UniProt Accession #: Q8WZ73
• NCBI Official Synonym Full Names: ring finger and FYVE like domain containing E3 ubiquitin protein ligase
• NCBI Official Symbol: RFFL
• NCBI Official Synonym Symbols: CARP2; FRING; CARP-2; RNF189; RNF34L; RIFIFYLIN
• NCBI Protein Information: E3 ubiquitin-protein ligase rififylin
• UniProt Protein Name: E3 ubiquitin-protein ligase rififylin
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: RFFL
• UniProt Synonym Gene Names: CARP-2; Fring
• UniProt Entry Name: RFFL_HUMAN

Uniprot Description

RFFL: Has E3 ubiquitin protein ligase activity. Regulates the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. Has anti-apoptotic activity. May bind phosphatidylinositol phosphates. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligase; EC 6.3.2.-; Vesicle; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: Golgi membrane; membrane; recycling endosome membrane; lysosome; cytoplasm; cytoplasmic membrane-bound vesicle; plasma membrane; endosome membrane; cytosol

Molecular Function: protein binding; protease binding; zinc ion binding; p53 binding; ubiquitin protein ligase binding; protein kinase binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; intracellular protein transport; proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination during ubiquitin-dependent protein catabolic process; regulation of TOR signaling pathway