Immunohistochemistry (IHC)
(Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NNF1R) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C.)
Rabbit NNF1R Polyclonal Antibody | anti-NNF1R antibody
NNF1R Polyclonal Antibody
Reactivity
Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep
Applications
Immunohistochemistry
Purity
affinity purified by Protein A
Synonyms
NNF1R; Polyclonal Antibody; NNF1R Polyclonal Antibody; PMF-1; Pmf1; PMF1_HUMAN; Polyamine modulated factor 1; Polyamine-modulated factor 1; anti-NNF1R antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep
Clonality
Polyclonal
Specificity
NNF1R Polyclonal Antibody detects endogenous levels of NNF1R protein.
Purity/Purification
affinity purified by Protein A
Form/Format
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Applicable Applications for anti-NNF1R antibody
Immunohistochemistry-Paraffin (IHC-P)
Application Notes
IHC-P: 1:100-500
Immunogen
KLH conjugated synthetic peptide derived from human NNF1R/PMF1:101-200/300
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.
Immunohistochemistry (IHC)
(Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NNF1R) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C.)
Immunohistochemistry (IHC)
(Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NNF1R) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C.)
Immunohistochemistry (IHC)
(Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NNF1R) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C.)
Immunohistochemistry (IHC)
(Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NNF1R) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C.)
Related Product Information for anti-NNF1R antibody
NNF1R, also called PMF1, is part of the MIS12 complex, which may be fundamental for kinetochore formation and proper chromosome segregation during mitosis. It is required for chromosome congression and for correct operation of the spindle checkpoint. May act as a cotranscription partner of NFE2L2 involved in regulation of polyamine-induced transcription of SSAT.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
20,230 Da
NCBI Official Full Name
polyamine-modulated factor 1 isoform 3
NCBI Official Synonym Full Names
polyamine modulated factor 1
NCBI Official Symbol
PMF1
NCBI Protein Information
polyamine-modulated factor 1
UniProt Protein Name
Polyamine-modulated factor 1
UniProt Gene Name
PMF1
UniProt Synonym Gene Names
PMF-1
Uniprot Description
Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis. May act as a cotranscription partner of NFE2L2 involved in regulation of polyamine-induced transcription of SSAT.
Research Articles on NNF1R
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Product Notes
The NNF1R pmf1 (Catalog #AAA3005624) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NNF1R Polyclonal Antibody reacts with Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's NNF1R can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin (IHC-P). IHC-P: 1:100-500. Researchers should empirically determine the suitability of the NNF1R pmf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NNF1R, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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