Rabbit RFC4 Polyclonal Antibody | anti-RFC4 antibody

RFC4 Antibody

Cat. Num. AAA5311925

• Gene Names: RFC4; A1; RFC37
• Applications: Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
• Purity: Antigen-Affinity Chromatography
• Synonyms: RFC4; Polyclonal Antibody; RFC4 Antibody; Affinity Purified Rabbit Polyclonal RFC4 Antibody; Polyclonal RFC4 antibody; Anti-RFC4 antibody; RFC37 antibody; RFC-4 antibody; RFC4 antibody; A1 antibody; RF-C 37 kDa subunit antibody; RFC 4; activator 1 37 kDa subunit antibody; MGC27291 antibody; A1 37 kDa subunit antibody; RFC-4; RFC 4 antibody; anti-RFC4 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen-Affinity Chromatography
• Form/Format: Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
• Concentration: 0.62 mg/ml (varies by lot)
• Sequence Length: 363

• Applicable Applications for anti-RFC4 antibody: Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IF: 1:100-1:500; ICC: 1:100-1:500; IHC-P: 1:100-1:500; WB: 1:500-1:3000
• Immunogen: RFC4 antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 18 and 323 of RFC4
• Cross-Reactivity: Human, Mouse
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of 50 ug of whole cell lysate (A: mouse liver) using a 10% SDS PAGE gel and RFC4 antibody at a dilution of 1:500)

Western Blot (WB)

(Western blot analysis of 30 ug of whole cell lysate (A: H1299; B: Hela; C: Hep G2) using a 10% SDS PAGE gel and RFC4 antibody at a dilution of 1:1000)

Immunofluorescence (IF)

(Immunofluorescence analysis of paraformaldehyde-fixed A549, using RFC4 antibody at 1:200 dilution.)

Immunohistochemistry (IHC)

(Immunohistochemical staining of paraffin-embedded MDAMB-468 Xenograft using RFC4 antibody at a dilution of 1:100)

Related Product Information for anti-RFC4 antibody

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system.

Product Categories/Family for anti-RFC4 antibody

Proteases; Inhibitors; & Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 49168606
• NCBI GeneID: 5984
• Molecular Weight: 40 kDa
• NCBI Official Full Name: RFC4
• NCBI Official Synonym Full Names: replication factor C subunit 4
• NCBI Official Symbol: RFC4
• NCBI Official Synonym Symbols: A1; RFC37
• NCBI Protein Information: replication factor C subunit 4
• Protein Family: Replication factor

NCBI Description

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]