Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, treated with Adriamycin (0.5ug/ml, 24hours), using RFA2 (Phospho-Thr21) antibody.)
Rabbit RFA2 Polyclonal Antibody | anti-RFA2 antibody
RFA2 antibody (Thr21)
Gene Names
RFA2; BUF1; RPA2; RPA32
Applications
Western Blot, Immunohistochemistry, ELISA
Synonyms
RFA2; Polyclonal Antibody; RFA2 antibody (Thr21); Polyclonal RFA2; Anti-RFA2; REPA2; Replication factor A protein 2; Replication protein A 32 kDa subunit; Replication protein A 34 kDa subunit; replication protein A2; 32kDa; RF-A protein 2; RP-A p32; RP-A p34; RPA2; RPA32; RPA34; anti-RFA2 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
RFA2 antibody detects endogenous levels of RFA2 only when phosphorylated at threonine 21
Form/Format
Supplied in PBS buffer (without Mg2+and Ca2+), pH 7.4, 150mM NaCl with 0.02% sodium azide and 50% glycerol
Concentration
1 mg/ml (varies by lot)
Sequence Length
273
Applicable Applications for anti-RFA2 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:100
ELISA: 1:1000
IHC: 1:50-1:100
ELISA: 1:1000
Biological Significance
RFA2 is required for DNA recombination, repair and replication.
Cross-Reactivity
Mouse
Immunogen
RFA2 antibody was raised in Rabbit using synthesized phosphopeptide derived from human RFA2 around the phosphorylation site of threonine 21 (G-Y-TP-Q-S) as the immunogen
Preparation and Storage
Aliquot and store at -20 degree C. Avoid repeated freeze thaw cycles
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, treated with Adriamycin (0.5ug/ml, 24hours), using RFA2 (Phospho-Thr21) antibody.)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, treated with Adriamycin (0.5ug/ml, 24hours), using RFA2 (Phospho-Thr21) antibody.)
Related Product Information for anti-RFA2 antibody
Rabbit polyclonal RFA2 antibody (Thr21)
Product Categories/Family for anti-RFA2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29,936 Da
NCBI Official Full Name
Rfa2p
NCBI Official Symbol
RFA2
NCBI Official Synonym Symbols
BUF1; RPA2; RPA32
NCBI Protein Information
Rfa2p
UniProt Protein Name
Replication factor A protein 2
Protein Family
UniProt Gene Name
RFA2
UniProt Synonym Gene Names
BUF1; RF-A protein 2
UniProt Entry Name
RFA2_YEAST
Uniprot Description
Binds to single-stranded sequences participating in DNA replication in addition to those mediating transcriptional repression (URS1) and activation (CAR1). Stimulates the activity of a cognate strand exchange protein (SEP1). It cooperates with T-AG and DNA topoisomerase I to unwind template DNA containing the simian virus 40 origin of DNA replication.
Research Articles on RFA2
Similar Products
Product Notes
The RFA2 rfa2 (Catalog #AAA5300376) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's RFA2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:1000 IHC: 1:50-1:100 ELISA: 1:1000. Researchers should empirically determine the suitability of the RFA2 rfa2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RFA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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