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Western Blot (WB) (Western blot analysis on purified restriction enzyme FokI.)

Rabbit Restriction Enzyme FokI Polyclonal Antibody | anti-fokIR antibody

Restriction Enzyme FokI (Internal) Antibody

Reactivity
Planomicrobium okeanokoites
Applications
Western Blot
Purity
Immunogen affinity purified
Synonyms
Restriction Enzyme FokI; Polyclonal Antibody; Restriction Enzyme FokI (Internal) Antibody; anti-fokIR antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Planomicrobium okeanokoites
Clonality
Polyclonal
Purity/Purification
Immunogen affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-fokIR antibody
Western Blot (WB)
Application Notes
WB: 1:1000-1:2000
Immunogen
Peptide
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.

Western Blot (WB)

(Western blot analysis on purified restriction enzyme FokI.)

Western Blot (WB) (Western blot analysis on purified restriction enzyme FokI.)
Related Product Information for anti-fokIR antibody
The enzyme FokI, naturally found in Flavobacterium okeanokoites, is a bacterial type IIS restriction endonuclease consisting of an N-terminal DNA-binding domain and a non-specific DNA cleavage domain at the C-terminal. Once the protein is bound to duplex DNA via its DNA-binding domain at the 5'-GGATG-3':3'-CATCC-5' recognition site, the DNA cleavage domain is activated and cleaves, without further sequence specificity, the first strand 9 nucleotides downstream and the second strand 13 nucleotides upstream of the nearest nucleotide of the recognition site. Its molecular mass is 65.4 kDa, being composed of 587 amino acids.
Product Categories/Family for anti-fokIR antibody

NCBI and Uniprot Product Information

NCBI GI #
UniProt Accession #
Molecular Weight
66kDa
NCBI Official Full Name
Type-2 restriction enzyme FokI
UniProt Protein Name
Type-2 restriction enzyme FokI
Protein Family
UniProt Gene Name
fokIR
UniProt Synonym Gene Names
rfoKI; R.FokI
UniProt Entry Name
T2F1_PLAOK

Uniprot Description

Recognizes the double-stranded sequence 5'-GGATG-3'/3'-CATCC-5' and cleaves respectively 14 bases after G-1 and 13 bases before C-1.

Similar Products

Product Notes

The Restriction Enzyme FokI fokir (Catalog #AAA9435090) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Restriction Enzyme FokI (Internal) Antibody reacts with Planomicrobium okeanokoites and may cross-react with other species as described in the data sheet. AAA Biotech's Restriction Enzyme FokI can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000-1:2000. Researchers should empirically determine the suitability of the Restriction Enzyme FokI fokir for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Restriction Enzyme FokI, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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