Rabbit anti-Human REST Polyclonal Antibody | anti-REST antibody

REST, ID (REST, NRSF, XBR, RE1-silencing transcription factor, Neural-restrictive silencer factor, X2 box repressor) (FITC)

Cat. Num. AAA6341337

• Gene Names: REST; WT6; XBR; NRSF
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: REST; Polyclonal Antibody; ID (REST; NRSF; XBR; RE1-silencing transcription factor; Neural-restrictive silencer factor; X2 box repressor) (FITC); X2 box repressor; anti-REST antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human REST.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-REST antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 542-570 in the central region of human human REST, UniProt Accession #Q13127; NP_005603.3; NP_001180437.1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in Jurkat cell line lysates (35ug/lane) using 040963. This demonstrates that 040963 detected the REST protein (arrow).)

Testing Data

(All lanes: 040963 (1:2000) Lane 1: Hela whole cell lysate (20ug) Lane 2: Jurkat whole cell lysate (20ug) Lane 3: PC-3 whole cell lysate (20ug) Lane 4: Raji whole cell lysate (20ug) Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 122kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Testing Data

(All lanes: 040963 (1:2000) Lane 1: 293T/17 whole cell lysate (20ug) Lane 2: Jurkat whole cell lysate (20ug) Lane 3: Raji whole cell lysate (20ug). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 122kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-REST antibody

Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier.

Product Categories/Family for anti-REST antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 301897974
• NCBI GeneID: 5978
• NCBI Accession #: NP_005603.3; NP_001180437.1
• NCBI GenBank Nucleotide #: NM_005612.4
• UniProt Accession #: Q13127; Q12956; Q12957; Q13134; Q59ER1; Q8IWI3; A2RUE0; B9EGJ0
• Molecular Weight: 119,083 Da
• NCBI Official Full Name: RE1-silencing transcription factor
• NCBI Official Synonym Full Names: RE1 silencing transcription factor
• NCBI Official Symbol: REST
• NCBI Official Synonym Symbols: WT6; XBR; NRSF
• NCBI Protein Information: RE1-silencing transcription factor
• UniProt Protein Name: RE1-silencing transcription factor
• Protein Family: Restin
• UniProt Gene Name: REST
• UniProt Synonym Gene Names: NRSF; XBR
• UniProt Entry Name: REST_HUMAN

NCBI Description

This gene encodes a transcriptional repressor that represses neuronal genes in non-neuronal tissues. It is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells and it is thought that this repressor may act as a master negative regular of neurogenesis. Alternatively spliced transcript variants have been described [provided by RefSeq, Jul 2010]