Western Blot (WB)
(Western blot analysis of extracts from HT-29 cells, using REQU antibody.)
Rabbit anti-Human, Mouse REQU Polyclonal Antibody | anti-REQU antibody
REQU Antibody
IF/ICC: 1:100-1:500
ELISA(peptide): 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
Western Blot (WB)
(Western blot analysis of extracts from HT-29 cells, using REQU antibody.)
Western Blot (WB)
(Western blot analysis of extracts from HT-29 cells, using REQU antibody.)
Immunofluorescence (IF)
(MBS9604841 staining HT29 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Immunofluorescence (IF)
(MBS9604841 staining HT29 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Similarity: Belongs to the requiem/DPF family.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the d4 domain family, characterized by a zinc finger-like structural motif. This protein functions as a transcription factor which is necessary for the apoptotic response following deprivation of survival factors. It likely serves a regulatory role in rapid hematopoietic cell growth and turnover. This gene is considered a candidate gene for multiple endocrine neoplasia type I, an inherited cancer syndrome involving multiple parathyroid, enteropancreatic, and pituitary tumors. [provided by RefSeq, Jul 2008]
Uniprot Description
May be a transcription factor required for the apoptosis response following survival factor withdrawal from myeloid cells. Might also have a role in the development and maturation of lymphoid cells.
Research Articles on REQU
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Product Notes
The REQU dpf2 (Catalog #AAA9604841) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The REQU Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's REQU can be used in a range of immunoassay formats including, but not limited to, Positive Control; Immunogen; SDS-PAGE; Western Blot (WB). WB: 1:500-1:1000 IF/ICC: 1:100-1:500 ELISA(peptide): 1:20000-1:40000 *The optimal dilutions should be determined by the end user. Researchers should empirically determine the suitability of the REQU dpf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "REQU, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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