Rabbit RELA / NFKB p65 Polyclonal Antibody | anti-RELA antibody

Rabbit Polyclonal to Human RELA / NFKB p65

Cat. Num. AAA240953

• Gene Names: RELA; p65; NFKB3
• Reactivity: Horse, Human, Monkey, Pig, Rabbit
• Applications: Immunohistochemistry, Western Blot, Immunoprecipitation, ELISA
• Purity: Delipidated and defibrinated
• Synonyms: RELA / NFKB p65; Polyclonal Antibody; Rabbit Polyclonal to Human RELA / NFKB p65; Anti-RELA / NFKB p65 Antibody (phospho-Ser529) IHC-plus; RELA; NF-kappa-B p65delta3; NFKB3; p65; Transcription factor p65; Human RELA; NFKB p65; anti-RELA antibody

• Host: Rabbit
• Reactivity: Horse, Human, Monkey, Pig, Rabbit
• Clonality: Polyclonal
• Specificity: NFkB p65 (Rel A) peptide corresponding to a region near phospho Serine 529 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH). Sequence information: PNGLLpSGDEDFC.
• Purity/Purification: Delipidated and defibrinated
• Form/Format: Serum, 0.1% sodium azide.
• Concentration: 75 mg/ml (varies by lot)
• Sequence Length: 551

• Applicable Applications for anti-RELA antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA)
• Application Notes: ELISA, IHC-P (1:500), IP, WB; Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
• Target Species: Human
• Antigen Modification: pSer529
• Preparation and Storage: +4 degree C or -20 degree C, Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-RELA antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.)

Immunohistochemistry (IHC)

(Anti-RELA antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.)

Western Blot (WB)

(Top row is anti-pan p65. Bottom row is anti-pS529 showing phospho p65 staining in carcinoma cells. Immunoblot of total protein lysates from various human head and neck tumors shows phospho p65 staining in tumor cell lines using phospho specific polyclonal anti-human pS529 p65. Lanes 1-6 contain protein lysates from human squamal carcinoma cell lines. Lane 7 is a protein lysate from a primary culture of human keratinocytes. Lane 8 contains protein lysate from #SCC9 cells (also a head and)

Western Blot (WB)

(Anti-pS529 shows phospho p65 staining in carcinoma cells.)

Western Blot (WB)

(Anti-Human pS276 p65 Antibody - Western Blot. TNF Induces phosphorylation of p65 in KBM-5 cells.)

Western Blot (WB)

(Anti-Human pS276 p65 Antibody - Western Blot. TNF Induces phosphorylation of p65 in KBM-5 cells. Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS276 p65. pan reactive anti-p65 was used a control to show the presence of total p65 in both the cytoplasmic and nuclear extracts. Phosphorylation of p65 occurs after approximately 10 min of TNF exposure. Migration of phosphorylated p65 into the nucleus occurs within a similar time frame. HRP conjugated Gt-anti-Rabbit IgG was used to develop the Western blot of a chemiluminescent detection method. Other detection methods will yield similar results. Personal Communication, Aggarwal BB.)

Related Product Information for anti-RELA antibody

NFKB1 or NFKB2 bind to REL, RELA, or RELB to form the NFKB complex. The NFKB1/RELA heterodimer is the most abundant form of NFKB. The NFKB complex is inactivated by I-kappa-B proteins that keep it trapped in the cytoplasm. Phosphorylation of serine residues on the I-kappa B proteins mark them for ubiquitination and destruction, and NFKB translocates to the nucleus where it binds DNA.

NCBI and Uniprot Product Information

• NCBI GI #: 223468676
• NCBI GeneID: 5970
• NCBI Accession #: NP_068810.3
• NCBI GenBank Nucleotide #: NM_021975.3
• UniProt Accession #: Q04206; Q6GTV1; Q6SLK1
• Molecular Weight: 59,910 Da
• NCBI Official Full Name: transcription factor p65 isoform 1
• NCBI Official Synonym Full Names: v-rel avian reticuloendotheliosis viral oncogene homolog A
• NCBI Official Symbol: RELA
• NCBI Official Synonym Symbols: p65; NFKB3
• NCBI Protein Information: transcription factor p65; NF-kappa-B p65delta3; nuclear factor NF-kappa-B p65 subunit; v-rel reticuloendotheliosis viral oncogene homolog A; nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
• UniProt Protein Name: Transcription factor p65
• Protein Family: Transcription factor
• UniProt Gene Name: RELA
• UniProt Synonym Gene Names: NFKB3
• UniProt Entry Name: TF65_HUMAN

NCBI Description

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

NFkB-p65: a subunit of NF-kappa-B transcription complex, which plays a crucial role in inflammatory and immune responses. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. P65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Three splice-variant isoforms have been identified.

Protein type: DNA-binding; Transcription factor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus; cytosol

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; identical protein binding; transcription activator binding; protein N-terminus binding; protein kinase binding; transcription factor binding; NF-kappaB binding; protein binding; DNA binding; protein heterodimerization activity; ubiquitin protein ligase binding; protein complex binding; chromatin binding; phosphate binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; response to cAMP; nerve growth factor receptor signaling pathway; viral reproduction; positive regulation of transcription, DNA-dependent; response to morphine; negative regulation of insulin receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter; toll-like receptor 3 signaling pathway; T cell receptor signaling pathway; toll-like receptor 10 signaling pathway; activation of NF-kappaB transcription factor; response to insulin stimulus; toll-like receptor 5 signaling pathway; response to organic substance; hair follicle development; positive regulation of cell proliferation; positive regulation of interferon type I production; inflammatory response; defense response to virus; toll-like receptor 4 signaling pathway; aging; response to drug; positive regulation of Schwann cell differentiation; membrane protein intracellular domain proteolysis; positive regulation of I-kappaB kinase/NF-kappaB cascade; cytokine and chemokine mediated signaling pathway; MyD88-independent toll-like receptor signaling pathway; acetaldehyde metabolic process; response to amino acid stimulus; liver development; toll-like receptor 2 signaling pathway; response to UV-B; MyD88-dependent toll-like receptor signaling pathway; organ morphogenesis; positive regulation of interleukin-12 biosynthetic process; positive regulation of chondrocyte differentiation; response to muramyl dipeptide; response to mechanical stimulus; regulation of inflammatory response; toll-like receptor signaling pathway; innate immune response; cellular defense response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; negative regulation of protein catabolic process; response to progesterone stimulus; negative regulation of transcription, DNA-dependent; response to cobalamin; negative regulation of apoptosis