Cadherin-12 / CDH12 Recombinant Protein | CDH12 recombinant protein

Recombinant Human Cadherin-12 / CDH12 Protein (His tag)

Cat. Num. AAA2546343

• Gene Names: CDH2; CDHN; NCAD; CD325; CDw325
• Purity: > 80 % as determined by SDS-PAGE
• Synonyms: Cadherin-12 / CDH12; Recombinant Human Cadherin-12 / CDH12 Protein (His tag); CDHB; FLJ34857; CDH12; CDH12 recombinant protein

• Host: Human Cells
• Purity/Purification: > 80 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM sodium citrate, 50mM NaCl, 2mM CaCl2, pH 6.0
• Sequence Length: 906

• Application Notes: The recombinant human CDH12 comprises 593 amino acids and has a predicted molecular mass of 66 kDa. The apparent molecular mass of rhCDH12 is approximately 80-85 kDa in SDS-PAGE due to glycosylation.
• Predicted N Terminal: Gln 24
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CDH12 recombinant protein

Background: Classic Cadherins represent a family of calcium-dependent homophilic cell-cell adhesion molecules. They confer strong adhesiveness to animal cells when they are anchored to the actin cytoskeleton via their cytoplasmic binding partners, catenins. The cadherin/catenin adhesion system plays key roles in the morphogenesis and function of the vertebrate and invertebrate nervous systems. Furthermore, this system is involved in synaptic plasticity. Recent studies on the role of individual cadherin subtype s at synapses indicate that individual cadherin subtype s play their own unique role to regulate synaptic activities. type II (atypical) cadherins are defined based on their lack of an HAV cell adhesion recognition sequence specific to type I cadherins. It has been observed that cells containing a specific cadherin subtype tend to cluster together to the exclusion of other type s, both in cell culture and during development. Cadherin-12 also known as CDH12, is a type II classical cadherin from the cadherin superfamily of integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Cadherin-12 appears to be expressed specifically in the brain and its temporal pattern of expression would be consistent with a role during a critical period of neuronal development, perhaps specifically during synaptogenesis.

Description: A DNA sequence encoding the pro form of human CDH12 (NP_001783.2) extracellular domain (Met 1-Ala 605) was fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 14589889
• NCBI GeneID: 1000
• NCBI Accession #: NP_001783.2
• NCBI GenBank Nucleotide #: NM_001792.4
• UniProt Accession #: P19022; Q14923; Q8N173; A8MWK3; B0YIY6
• Molecular Weight: 97,040 Da
• NCBI Official Full Name: cadherin-2 isoform 1 preproprotein
• NCBI Official Synonym Full Names: cadherin 2
• NCBI Official Symbol: CDH2
• NCBI Official Synonym Symbols: CDHN; NCAD; CD325; CDw325
• NCBI Protein Information: cadherin-2
• UniProt Protein Name: Cadherin-2
• Protein Family: Cadherin
• UniProt Gene Name: CDH2
• UniProt Synonym Gene Names: CDHN; NCAD; N-cadherin

NCBI Description

This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone. [provided by RefSeq, Nov 2015]

Uniprot Description

CDH2: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. Interacts with CDCP1. Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. Interacts with PCDH8; this complex may also include TAOK2. The interaction with PCDH8 may lead to internalization through TAOK2/p38 MAPK pathway.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 18q12.1

Cellular Component: catenin complex; cell-cell adherens junction; cortical actin cytoskeleton; cytoplasm; focal adhesion; intercellular junction; lamellipodium; plasma membrane

Molecular Function: alpha-catenin binding; beta-catenin binding; gamma-catenin binding; protein binding

Biological Process: cell adhesion; glial cell differentiation; positive regulation of muscle cell differentiation