Mouse Regenerating Islet Derived Protein 3 Gamma (REG3g) ELISA Kit | REG3g elisa kit

Mouse Regenerating Islet Derived Protein 3 Gamma (REG3g) ELISA Kit

Cat. Num. AAA451182

• Gene Names: Reg3g; AI449515; REG-3-gamma; reg III-gamma
• Reactivity: Mouse
• Synonyms: Regenerating Islet Derived Protein 3 Gamma (REG3g); Mouse Regenerating Islet Derived Protein 3 Gamma (REG3g) ELISA Kit; REG-III; PAP1B; PAPIB; Pancreatitis-associated protein 1B; Regenerating islet-derived protein III-gamma; REG3g elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of REG3g. No significant cross-reactivity or interference between REG3g and analogues was observed.
• Sequence Length: 174

• Samples: Serum, plasma or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 15.6-1000pg/mL
• Sensitivity: < 6.9pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level REG3g were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level REG3g were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for REG3g elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of REG3g in mouse serum, plasma or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to REG3g. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to REG3g. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain REG3g, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of REG3g in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6755310
• NCBI GeneID: 19695
• NCBI Accession #: NP_035390.1
• NCBI GenBank Nucleotide #: NM_011260.2
• UniProt Accession #: O09049
• Molecular Weight: 19,307 Da
• NCBI Official Full Name: regenerating islet-derived protein 3-gamma preproprotein
• NCBI Official Synonym Full Names: regenerating islet-derived 3 gamma
• NCBI Official Symbol: Reg3g
• NCBI Official Synonym Symbols: AI449515; REG-3-gamma; reg III-gamma
• NCBI Protein Information: regenerating islet-derived protein 3-gamma
• UniProt Protein Name: Regenerating islet-derived protein 3-gamma
• Protein Family: Regenerating islet-derived protein
• UniProt Gene Name: Reg3g
• UniProt Synonym Gene Names: Pap3; REG-3-gamma; Reg III-gamma

NCBI Description

This gene encodes a C-type lectin that demonstrates bactericidal activity. This gene is predominantly expressed in the distal small intestine where the encoded protein undergoes proteolytic processing by trypsin. Mice lacking the encoded protein exhibit altered mucus distribution, increased bacterial contact with the epithelium, and elevated inflammatory markers in the ileum, and low-grade inflammation. [provided by RefSeq, Jun 2016]

Uniprot Description

Bactericidal C-type lectin which acts exclusively against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan. Restricts bacterial colonization of the intestinal epithelial surface and consequently limits activation of adaptive immune responses by the microbiota. The uncleaved form has bacteriostatic activity, whereas the cleaved form has bactericidal activity against L.monocytogenes and methicillin-resistant S.aureus. Regulates keratinocyte proliferation and differentiation after skin injury.