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Flow Cytometry (FC/FACS) (Flow cytometric analysis of HepG2 cells using REG3A Antibody (C-term)(green) compared to an isotype control of rabbit IgG(blue). MBS9208338 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)

Rabbit anti-Human REG3A Polyclonal Antibody | anti-REG3A antibody

REG3A Antibody (C-term)

Gene Names
REG3A; HIP; PAP; PAP1; REG3; INGAP; PAP-H; PBCGF; HIP/PAP; REG-III
Reactivity
Human
Applications
Western Blot, ELISA, Flow Cytometry, Functional Assay
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
REG3A; Polyclonal Antibody; REG3A Antibody (C-term); Regenerating islet-derived protein 3-alpha; REG-3-alpha; Hepatointestinal pancreatic protein; HIP/PAP; Human proislet peptide; Pancreatitis-associated protein 1; Regenerating islet-derived protein III-alpha; Reg III-alpha; Regenerating islet-derived protein 3-alpha 165 kDa form; Regenerating islet-derived protein 3-alpha 15 kDa form; HIP; PAP; PAP1; anti-REG3A antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This REG3A antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 129-163 amino acids from the C-terminal region of human REG3A.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
175
Applicable Applications for anti-REG3A antibody
Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)
Application Notes
FC~~1:25
WB~~1:1000
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of HepG2 cells using REG3A Antibody (C-term)(green) compared to an isotype control of rabbit IgG(blue). MBS9208338 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)

Flow Cytometry (FC/FACS) (Flow cytometric analysis of HepG2 cells using REG3A Antibody (C-term)(green) compared to an isotype control of rabbit IgG(blue). MBS9208338 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)

Western Blot (WB)

(Western blot analysis of lysate from human pancreas tissue lysate, using REG3A Antibody (C-term). MBS9208338 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)

Western Blot (WB) (Western blot analysis of lysate from human pancreas tissue lysate, using REG3A Antibody (C-term). MBS9208338 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
Related Product Information for anti-REG3A antibody
Bactericidal C-type lectin which acts exclusively against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan. Regulates keratinocyte proliferation and differentiation after skin injury via activation of EXTL3-PI3K-AKT signaling pathway.
Product Categories/Family for anti-REG3A antibody
References
Itoh T.,et al.Biochim. Biophys. Acta 1172:184-186(1993).
Orelle B.,et al.J. Clin. Invest. 90:2284-2291(1992).
Lasserre C.,et al.Cancer Res. 52:5089-5095(1992).
Dusetti N.J.,et al.Genomics 19:108-114(1994).
Lasserre C.,et al.Eur. J. Biochem. 224:29-38(1994).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
19395
NCBI Official Full Name
regenerating islet-derived protein 3-alpha
NCBI Official Synonym Full Names
regenerating islet-derived 3 alpha
NCBI Official Symbol
REG3A
NCBI Official Synonym Symbols
HIP; PAP; PAP1; REG3; INGAP; PAP-H; PBCGF; HIP/PAP; REG-III
NCBI Protein Information
regenerating islet-derived protein 3-alpha
UniProt Protein Name
Regenerating islet-derived protein 3-alpha
UniProt Gene Name
REG3A
UniProt Synonym Gene Names
HIP; PAP; PAP1; REG-3-alpha; HIP/PAP; Reg III-alpha
UniProt Entry Name
REG3A_HUMAN

NCBI Description

This gene encodes a pancreatic secretory protein that may be involved in cell proliferation or differentiation. It has similarity to the C-type lectin superfamily. The enhanced expression of this gene is observed during pancreatic inflammation and liver carcinogenesis. The mature protein also functions as an antimicrobial protein with antibacterial activity. Alternate splicing results in multiple transcript variants that encode the same protein.[provided by RefSeq, Nov 2014]

Uniprot Description

REG3A: Might be a stress protein involved in the control of bacterial proliferation.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 2p12

Cellular Component: extracellular space; cytoplasm

Molecular Function: carbohydrate binding

Biological Process: heterophilic cell adhesion; cell proliferation; multicellular organismal development; negative regulation of keratinocyte differentiation; acute-phase response

Research Articles on REG3A

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Product Notes

The REG3A reg3a (Catalog #AAA9208338) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The REG3A Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's REG3A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS). FC~~1:25 WB~~1:1000. Researchers should empirically determine the suitability of the REG3A reg3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "REG3A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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