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Immunohistochemistry (IHC) (Immunochemical staining of human REG3A in human small intestine with rabbit polyclonal antibody at 1:10000 dilution, formalin-fixed paraffin embedded sections.)

Rabbit anti-Human Reg3A Polyclonal Antibody | anti-REG3A antibody

Anti-Reg3A Antibody, Rabbit Polyclonal

Gene Names
REG3A; HIP; PAP; PAP1; REG3; INGAP; PAP-H; PBCGF; HIP/PAP; REG-III
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
Protein A & Antigen Affinity
Synonyms
Reg3A; Polyclonal Antibody; Anti-Reg3A Antibody; Rabbit Polyclonal; REG3A Antibody; Rabbit PAb; Antigen Affinity Purified; regenerating family member 3 alpha; Anti-HIP Antibody; Anti-HIP/PAP Antibody; Anti-INGAP Antibody; Anti-PAP Antibody; Anti-PAP-H Antibody; Anti-PAP1 Antibody; Anti-PBCGF Antibody; Anti-REG-III Antibody; Anti-REG3 Antibody; Anti-REG3A Antibody; Anti-REG3V Antibody; anti-REG3A antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human REG3A
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-REG3A antibody
ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
ELISA: 1:5000-1:10,000
IHC-P: 1:1000-1:20,000
ICC/IF: 1:300-1:10,000
Immunogen
Recombinant Human REG3A Protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human REG3A (rh REG3A). REG3A specific IgG was purified by Human REG3A affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human REG3A in human small intestine with rabbit polyclonal antibody at 1:10000 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC) (Immunochemical staining of human REG3A in human small intestine with rabbit polyclonal antibody at 1:10000 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human REG3A in human pancreatic carcinoma with rabbit polyclonal antibody at 1:10000 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC) (Immunochemical staining of human REG3A in human pancreatic carcinoma with rabbit polyclonal antibody at 1:10000 dilution, formalin-fixed paraffin embedded sections.)

Immunofluorescence (IF)

(Immunofluorescence staining of REG3A-His in HT29 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti- REG3A-His polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to cytoplasm.)

Immunofluorescence (IF) (Immunofluorescence staining of REG3A-His in HT29 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti- REG3A-His polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to cytoplasm.)
Related Product Information for anti-REG3A antibody
Regenerating islet-derived protein 3-alpha, also known as Regenerating islet-derived protein III-alpha, REG-3-alpha, REG3A, and HIP, is secreted protein that contains one C-type lectin domain. REG3A is constitutively expressed in intestine, and is a pancreatic secretory protein that may be involved in cell proliferation or differentiation. It is overexpressed during the acute phase of pancreatitis and in some patients with chronic pancreatitis. REG3A and REG1A proteins are both involved in liver and pancreatic regeneration and proliferation. REG3A is also a stress protein involved in the control of bacterial proliferation. REG3A is down-regulated in most primary human gastric cancer cells, and might be useful in the identification of gastric cancer. Additionally, REG3A is a target of beta-catenin signaling in Huh7 hepatoma cells. The REG1A and REG3A are downstream targets of the Wnt pathway during liver tumorigenesis.
References
Cavard C, et al. (2006) Overexpression of regenerating islet-derived 1 alpha and 3 alpha genes in human primary liver tumors with beta-catenin mutations. Oncogene. 25(4): 599-608.Choi B, et al. (2007) Downregulation of regenerating islet-derived 3 alpha (REG3A) in primary human gastric adenocarcinomas. Exp Mol Med. 39(6): 796-804.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
19,395 Da
NCBI Official Full Name
regenerating islet-derived protein 3-alpha
NCBI Official Synonym Full Names
regenerating islet-derived 3 alpha
NCBI Official Symbol
REG3A
NCBI Official Synonym Symbols
HIP; PAP; PAP1; REG3; INGAP; PAP-H; PBCGF; HIP/PAP; REG-III
NCBI Protein Information
regenerating islet-derived protein 3-alpha; REG-3-alpha; reg III-alpha; PAP homologous protein; human proislet peptide; pancreatitis-associated protein 1; proliferation-inducing protein 34; proliferation-inducing protein 42; hepatocarcinoma-intestine-panc
UniProt Protein Name
Regenerating islet-derived protein 3-alpha
UniProt Gene Name
REG3A
UniProt Synonym Gene Names
HIP; PAP; PAP1; REG-3-alpha; HIP/PAP
UniProt Entry Name
REG3A_HUMAN

NCBI Description

This gene encodes a pancreatic secretory protein that may be involved in cell proliferation or differentiation. It has similarity to the C-type lectin superfamily. The enhanced expression of this gene is observed during pancreatic inflammation and liver carcinogenesis. Alternate splicing results in multiple transcript variants that encode the same protein.[provided by RefSeq, Dec 2010]

Uniprot Description

REG3A: Might be a stress protein involved in the control of bacterial proliferation.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2p12

Cellular Component: extracellular space; cytoplasm

Molecular Function: carbohydrate binding

Biological Process: heterophilic cell adhesion; cell proliferation; multicellular organismal development; negative regulation of keratinocyte differentiation; acute-phase response

Research Articles on REG3A

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Product Notes

The REG3A reg3a (Catalog #AAA8105148) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Reg3A Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Reg3A can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF). ELISA: 1:5000-1:10,000 IHC-P: 1:1000-1:20,000 ICC/IF: 1:300-1:10,000. Researchers should empirically determine the suitability of the REG3A reg3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Reg3A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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