Rabbit anti-Human MAT1A Polyclonal Antibody | anti-MAT1A antibody

MAT1A, NT (MAT1A, AMS1, MATA1, S-adenosylmethionine synthase isoform type-1, Methionine adenosyltransferase 1, Methionine adenosyltransferase I/III) (PE)

Cat. Num. AAA6319154

• Gene Names: MAT1A; MAT; SAMS; MATA1; SAMS1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MAT1A; Polyclonal Antibody; NT (MAT1A; AMS1; MATA1; S-adenosylmethionine synthase isoform type-1; Methionine adenosyltransferase 1; Methionine adenosyltransferase I/III) (PE); Methionine adenosyltransferase I/III; anti-MAT1A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MAT1A
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-MAT1A antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 107-136 in the N-terminal region of human MAT1A, UniProt Accession #Q00266. Species sequence homology: bovine, mouse and rat
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in MDA-MB435 cell line lysates (35ug/lane) usingMBS646747. This demonstrates the MAT1A antibody detected the MAT1A protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue using MBS646747 followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Related Product Information for anti-MAT1A antibody

This gene catalyzes a two-step reaction that involves the transfer of the adenosyl moiety of ATP to methionine to form S-adenosylmethionine and tripolyphosphate, which is subsequently cleaved to PPi and Pi. S-adenosylmethionine is the source of methyl groups for most biological methylations. The encoded protein is found as a homotetramer (MAT I) or a homodimer (MAT III) whereas a third form, MAT II (gamma), is encoded by the MAT2A gene. Mutations in this gene are associated with methionine adenosyltransferase deficiency.

Product Categories/Family for anti-MAT1A antibody

Antibodies; Enzymes; Synthase

References

1. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). 2. Wu, S.M., et al. Cell. Mol. Life Sci. 67(11):1831-1843(2010). 3. Lai, C.Q., et al. Am. J. Clin. Nutr. 91(5):1377-1386(2010). 4. Joslyn, G., et al. Alcohol. Clin. Exp. Res. 34(5):800-812(2010). 5. Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 4557737
• NCBI GeneID: 4143
• NCBI Accession #: NP_000420.1
• NCBI GenBank Nucleotide #: NM_000429.2
• UniProt Accession #: Q00266; Q5QP09; D3DWD5
• Molecular Weight: 395
• NCBI Official Full Name: S-adenosylmethionine synthase isoform type-1
• NCBI Official Synonym Full Names: methionine adenosyltransferase I, alpha
• NCBI Official Symbol: MAT1A
• NCBI Official Synonym Symbols: MAT; SAMS; MATA1; SAMS1
• NCBI Protein Information: S-adenosylmethionine synthase isoform type-1; MAT 1; MAT-I/III; adoMet synthase 1; adoMet synthetase 1; methionine adenosyltransferase 1; methionine adenosyltransferase I/III; S-adenosylmethionine synthetase isoform type-1
• UniProt Protein Name: S-adenosylmethionine synthase isoform type-1
• Protein Family: S-adenosylmethionine synthase
• UniProt Gene Name: MAT1A
• UniProt Synonym Gene Names: AMS1; MATA1; AdoMet synthase 1; MAT 1; MAT-I/III
• UniProt Entry Name: METK1_HUMAN