Western Blot (WB)
(Anti-REA antibody, MBS176293, Western blottingLane 1: PANC Cell LysateLane 2: COLO320 Cell LysateLane 3: U87Cell LysateLane 4: HEPA Cell Lysate )
Rabbit REA Polyclonal Antibody | anti-PHB2 antibody
Anti-REA antibody
Gene Names
PHB2; BAP; REA; p22; Bap37; BCAP37; PNAS-141
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
Immunogen affinity purified.
Synonyms
REA; Polyclonal Antibody; Anti-REA antibody; Prohibitin-2; prohibitin 2; B cell receptor associated protein BAP37 antibody; B-cell associated protein antibody; B-cell receptor-associated protein BAP37 antibody; Bap 37 antibody; BAP antibody; Bap37 antibody; BCAP 37 antibody; BCAP37 antibody; D prohibitin antibody; D-prohibitin antibody; MGC117268 antibody; p22 antibody; PHB 2 antibody; PHB2 antibody; PHB2_HUMAN antibody; PNAS 141 antibody; PNAS141 antibody; Prohibitin 2 antibody; Prohibitin-2 antibody; Prohibitin2 antibody; Repressor of Estrogen Receptor Activity antibody; anti-PHB2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
299
Applicable Applications for anti-PHB2 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC)
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human REA (111-128aa RPNAQELPSMYQRLGLDY), identical to the related rat and mouse sequences.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Anti-REA antibody, MBS176293, Western blottingLane 1: PANC Cell LysateLane 2: COLO320 Cell LysateLane 3: U87Cell LysateLane 4: HEPA Cell Lysate )
Western Blot (WB)
(Anti-REA antibody, MBS176293, Western blottingLane 1: PANC Cell LysateLane 2: COLO320 Cell LysateLane 3: U87Cell LysateLane 4: HEPA Cell Lysate )
Immunohistochemistry (IHC)
(Anti-REA antibody, MBS176293, IHC(P)IHC(P): Human Lung Cancer Tissue )
Immunohistochemistry (IHC)
(Anti-REA antibody, MBS176293, IHC(P)IHC(P): Human Lung Cancer Tissue )
Immunocytochemistry (ICC)
(Anti-REA antibody, MBS176293, ICCICC: A549 Cell )
Immunocytochemistry (ICC)
(Anti-REA antibody, MBS176293, ICCICC: A549 Cell )
Related Product Information for anti-PHB2 antibody
Description: Rabbit IgG polyclonal antibody for Prohibitin-2(PHB2) detection. Tested with WB, IHC-P, ICC in Human, Mouse, Rat.
Background: PHB2(Prohibitin 2), also called Repressor of Estrogen Receptor Activity(REA), is a protein that in humans is encoded by the PHB2 gene. The International Radiation Hybrid Mapping Consortium mapped the PHB2 gene to chromosome 12. Montano et al.(1999) showed that REA enhanced the potency of a dominant-negative ER-alpha mutant and antiestrogens as suppressors of ER-alpha activity in Chinese hamster ovary cells. When coexpressed with wildtype ER-alpha or ER-beta(ESR2), REA suppressed activation of a reporter gene in a dose-dependent manner. REA had no effect on reporter activity in the absence of liganded ER, and it had no effect on the transcriptional activities of other hormone receptors. Mutation analysis showed that an N-terminal domain and a central domain of REA were required for its repressor activity.
Background: PHB2(Prohibitin 2), also called Repressor of Estrogen Receptor Activity(REA), is a protein that in humans is encoded by the PHB2 gene. The International Radiation Hybrid Mapping Consortium mapped the PHB2 gene to chromosome 12. Montano et al.(1999) showed that REA enhanced the potency of a dominant-negative ER-alpha mutant and antiestrogens as suppressors of ER-alpha activity in Chinese hamster ovary cells. When coexpressed with wildtype ER-alpha or ER-beta(ESR2), REA suppressed activation of a reporter gene in a dose-dependent manner. REA had no effect on reporter activity in the absence of liganded ER, and it had no effect on the transcriptional activities of other hormone receptors. Mutation analysis showed that an N-terminal domain and a central domain of REA were required for its repressor activity.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
29,044 Da
NCBI Official Full Name
Prohibitin-2
NCBI Official Synonym Full Names
prohibitin 2
NCBI Official Symbol
PHB2
NCBI Official Synonym Symbols
BAP; REA; p22; Bap37; BCAP37; PNAS-141
NCBI Protein Information
prohibitin-2; D-prohibitin; B-cell associated protein; repressor of estrogen receptor activity; B-cell receptor-associated protein BAP37; PHB2 {ECO:0000312|EMBL:AAH14766.1, ECO:0000312|HGNC:HGNC:30306}
UniProt Protein Name
Prohibitin-2
Protein Family
UniProt Gene Name
PHB2
UniProt Entry Name
PHB2_HUMAN
Uniprot Description
BAP37: a protein of the band 7 family. This family includes the prohibitins, cytoplasmic anti-proliferative proteins and stomatin, an erythrocyte membrane protein. A repressor of estrogen receptor (ER) activity. Reverses steroid receptor coactivator 1 enhancement of ER activity. May play a role in determining the sensitivity of estrogen target cells to antiestrogen and estrogen therapy.
Protein type: Mitochondrial; Transcription, coactivator/corepressor; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 12p13
Cellular Component: nuclear matrix; protein complex; mitochondrion; mitochondrial inner membrane; nucleus
Molecular Function: protein binding; estrogen receptor binding
Biological Process: transcription, DNA-dependent; negative regulation of transcription, DNA-dependent; negative regulation of mammary gland epithelial cell proliferation; negative regulation of estrogen receptor signaling pathway
Research Articles on PHB2
Similar Products
Product Notes
The PHB2 phb2 (Catalog #AAA176293) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-REA antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's REA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC). Researchers should empirically determine the suitability of the PHB2 phb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "REA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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