Rabbit AMPK beta 1 Polyclonal Antibody | anti-AMPK beta 1 antibody

Anti-AMPK beta 1 Antibody

Cat. Num. AAA415813

• Gene Names: PRKAB1; AMPK; HAMPKb
• Reactivity: Human. Not yet tested in other species
• Applications: ELISA, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: AMPK beta 1; Polyclonal Antibody; Anti-AMPK beta 1 Antibody; anti-AMPK beta 1 antibody

• Host: Rabbit
• Reactivity: Human. Not yet tested in other species
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 270

• Applicable Applications for anti-AMPK beta 1 antibody: ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: Peptide ELISA titer: 1:5,000-1:20,000; Immunohistochemistry: 1:100-500; Optimal dilutions/concentrations should be determined by the end user
• Storage Buffer: PBS, pH 7.4 with 0.02% Sodium Azide
• Immunogen: Rabbit polyclonal AMPK beta 1 antibody was raised against a 15 amino acid peptide from near the N terminal residues of human AMPK beta 1
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Immunohistochemistry (IHC)

References

(1) Woods,A., Cheung,P.C., Smith,F.C., Characterization of AMP-activated protein kinase beta and gamma subunits. Assembly of the heterotrimeric complex in vitro. Biochem J. 2000; 346(3):659-669.

NCBI and Uniprot Product Information

• NCBI GI #: 2916800
• NCBI GeneID: 5564
• UniProt Accession #: Q9Y478; Q9UBV0; Q9UE20; Q9UEX2; Q9Y6V8
• Molecular Weight: 30,382 Da
• NCBI Official Full Name: AMP-activated protein kinase, beta 1 subunit
• NCBI Official Synonym Full Names: protein kinase, AMP-activated, beta 1 non-catalytic subunit
• NCBI Official Symbol: PRKAB1
• NCBI Official Synonym Symbols: AMPK; HAMPKb
• NCBI Protein Information: 5'-AMP-activated protein kinase subunit beta-1; 5'-AMP-activated protein kinase beta-1 subunit; AMP-activated protein kinase beta subunit; AMPK beta -1 chain; AMPK beta 1; AMPK subunit beta-1; AMPKb; protein kinase, AMP-activated, noncatalytic, beta-1
• UniProt Protein Name: 5'-AMP-activated protein kinase subunit beta-1
• UniProt Gene Name: PRKAB1
• UniProt Entry Name: AAKB1_HUMAN

NCBI Description

The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex. [provided by RefSeq, Jul 2008]

Uniprot Description

AMPKB1: a non-catalytic subunit of AMPK, a conserved kinase of the CAMKL family. AMPK is an energy-sensing protein that plays a key role in regulating cellular energy homeostasis. Environmental stress, such as heat shock, nutrient deprivation, hypoxia and ischemia, indirectly activate AMPK by the depletion of cellular ATP and the concomitant rise of ADP and AMP levels. Allosteric activation is achieved primarily by rising ADP levels, and not solely by AMP levels as previously thought. Activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, probably by indirectly activating myosin. AMPK is a heterotrimer of an alpha catalytic subunit (AMPKA1 or -2), a beta (AMPKB1 or -2) and a gamma non-catalytic subunit (AMPKG1, -2 or -3). Different possible combinations of subunits give rise to 12 different holoenzymes. Beta subunits act as scaffolds on which the AMPK complex assembles, via its C-terminus that bridges alpha and gamma subunits. AMPK-beta1 or -beta2 subunits are required for assembling of AMPK heterotrimers and are important for regulating enzyme activity and cellular localization. AMPK beta1beta2 null mouse muscles reveal an essential role for AMPK in maintaining mitochondrial content and glucose uptake during exercise. Phosphorylation by ULK1 and ULK2 inhibits AMPK activity. Hematopoietic AMPKB1 reduces mouse adipose tissue macrophage inflammation and insulin resistance in obesity.

Protein type: Autophagy; Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 12q24.1-q24.3

Cellular Component: cytosol; nucleus; AMP-activated protein kinase complex

Molecular Function: AMP-activated protein kinase activity; protein binding; protein kinase binding; protein kinase activity

Biological Process: mitochondrion organization and biogenesis; protein heterooligomerization; organelle organization and biogenesis; insulin receptor signaling pathway; cell cycle arrest; regulation of protein kinase activity; signal transduction; protein amino acid phosphorylation; fatty acid biosynthetic process