Rabbit RBPJK/RBPJ Polyclonal Antibody | anti-RBPJ antibody

Anti-RBPJK/RBPJ Antibody

Cat. Num. AAA1752953

• Gene Names: RBPJ; SUH; csl; AOS3; CBF1; KBF2; CBF-1; RBP-J; RBPJK; IGKJRB; RBP-JK; RBPSUH; IGKJRB1; RBP-J kappa
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Flow Cytometry, Functional Assay, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: RBPJK/RBPJ; Polyclonal Antibody; Anti-RBPJK/RBPJ Antibody; Recombining binding protein suppressor of hairless; CBF-1; J kappa-recombination signal-binding protein; RBP-J kappa; RBP-J; RBP-JK; Renal carcinoma antigen NY-REN-30; RBPJ; IGKJRB; IGKJRB1; RBPJK; RBPSUH; anti-RBPJ antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 500

• Applicable Applications for anti-RBPJ antibody: Western Blot (WB), Flow Cytometry (FC/FACS), Direct ELISA (EIA)
• Application Notes: WB: 0.25-0.5ug/ml (Human, Mouse, Rat); FC/FACS: 1-3ug/1x106 cells (Human); Direct ELISA: 0.1-0.5ug/ml (Human); Tested Species: In-house tested species with positive results.
• Immunogen: E Coli-derived human RBPJK/RBPJ recombinant protein (Position: K41-Q467).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Flow Cytometry (FC/FACS)

Western Blot (WB)

(Figure 1. Western blot analysis of RBPJ using anti-RBPJ antibody.)

Related Product Information for anti-RBPJ antibody

Description: Rabbit IgG polyclonal antibody for RBPJK/RBPJ detection. Tested with WB, FCM, Direct ELISA in Human; Mouse; Rat.

Background: Recombination signal binding protein for immunoglobulin kappa J region is a protein that in humans is encoded by the RBPJ gene. It is mapped to 4p15.2. The protein encoded by this gene is a transcriptional regulator important in the Notch signaling pathway. The encoded protein acts as a repressor when not bound to Notch proteins and an activator when bound to Notch proteins. It is thought to function by recruiting chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins to Notch signaling pathway genes. Several transcript variants encoding different isoforms have been found for this gene, and several pseudogenes of this gene exist on chromosome 9.

References

1. Hassed, S. J., Wiley, G. B., Wang, S., Lee, J.-Y., Li, S., Xu, W., Zhao, Z. J., Mulvihill, J. J., Robertson, J., Warner, J., Gaffney, P. M. RBPJ mutations identified in two families affected by Adams-Oliver syndrome. Am. J. Hum. Genet. 91: 391-395, 2012.
2. Kulic, I., Robertson, G., Chang, L., Baker, J. H. E., Lockwood, W. W., Mok, W., Fuller, M., Fournier, M., Wong, N., Chou, V., Robinson, M. D., Chun, H.-J., and 9 others. Loss of the Notch effector RBPJ promotes tumorigenesis. J. Exp. Med. 212: 37-52, 2015.
3. Masui, T., Long, Q., Beres, T. M., Magnuson, M. A., MacDonald, R. J. Early pancreatic development requires the vertebrate suppressor of hairless (RBPJ) in the PTF1 bHLH complex. Genes Dev. 21: 2629-2643, 2007.

NCBI and Uniprot Product Information

• NCBI GI #: 42560227
• NCBI GeneID: 3516
• NCBI Accession #: NP_005340.2
• NCBI GenBank Nucleotide #: NP_005340.2
• UniProt Accession #: Q06330
• NCBI Official Full Name: recombining binding protein suppressor of hairless isoform 1
• NCBI Official Synonym Full Names: recombination signal binding protein for immunoglobulin kappa J region
• NCBI Official Symbol: RBPJ
• NCBI Official Synonym Symbols: SUH; csl; AOS3; CBF1; KBF2; CBF-1; RBP-J; RBPJK; IGKJRB; RBP-JK; RBPSUH; IGKJRB1; RBP-J kappa
• NCBI Protein Information: recombining binding protein suppressor of hairless
• UniProt Protein Name: Recombining binding protein suppressor of hairless
• Protein Family: Recombining binding protein suppressor of hairless
• UniProt Gene Name: RBPJ
• UniProt Synonym Gene Names: IGKJRB; IGKJRB1; RBPJK; RBPSUH; RBP-J; RBP-JK
• UniProt Entry Name: SUH_HUMAN

NCBI Description

The protein encoded by this gene is a transcriptional regulator important in the Notch signaling pathway. The encoded protein acts as a repressor when not bound to Notch proteins and an activator when bound to Notch proteins. It is thought to function by recruiting chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins to Notch signaling pathway genes. Several transcript variants encoding different isoforms have been found for this gene, and several pseudogenes of this gene exist on chromosome 9. [provided by RefSeq, Oct 2013]