Rabbit SNAP25 Polyclonal Antibody | anti-SNAP25 antibody

SNAP25 Antibody

Cat. Num. AAA9606828

• Gene Names: SNAP25; SUP; RIC4; SEC9; SNAP; CMS18; RIC-4; SNAP-25; bA416N4.2; dJ1068F16.2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: SNAP25; Polyclonal Antibody; SNAP25 Antibody; bA416N4.2; Bdr; CMS18; dJ1068F16.2; FLJ23079; HGNC:11132; MGC105414; MGC139754; Resistance to inhibitors of cholinesterase 4 homolog; RIC 4; RIC4; SEC 9; SEC9; SNAP 25; SNAP; SNAP-25; SNAP-25B; SNP 25; SNP25; SNP25_HUMAN; sp; SUP; Super protein; Synaptosomal associated 25 kDa protein; Synaptosomal associated protein; Synaptosomal associated protein 25; Synaptosomal associated protein 25kDa; Synaptosomal-associated 25 kDa protein; Synaptosomal-associated protein 25; Synaptosomal-associated protein; 25-KD; anti-SNAP25 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SNAP25 antibody detects endogenous levels of total SNAP25
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 206

• Applicable Applications for anti-SNAP25 antibody: Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF/ICC: 1:100-1:500
• Immunogen: A synthesized peptide derived from human SNAP25
• Subcellular Location: Cytoplasm > Perinuclear Region. Cell Membrane. Cell Junction > Synapse > Synaptosome. Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region.
• Tissue Specificity: Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Immunohistochemistry (IHC)

(MBS9606828 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Immunofluorescene (IF)

(MBS9606828 staining RAW264.7 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

Western Blot (WB)

(Western blot analysis of SNAP25 expression in various lysates)

Related Product Information for anti-SNAP25 antibody

Description: The 25 kDasynaptosome-associated protein (SNAP25) is a target membrane soluble, N-ethylmaleimide-sensitive factor attachment protein receptor (t-SNARE) that is found on neuronal presynaptic membranes. SNAP25 forms a core complex with the SNARE proteins syntaxin and synaptobrevin to mediate synaptic vesicle fusion with the plasma membrane during Ca2+-dependent exocytosis (1). This complex is responsible for exocytosis of the neurotransmitter gamma-aminobutyric acid (GABA). Neurotransmitter release is inhibited by proteolysis of SNAP25 by botulinum toxins A and E (2). SNAP25 plays a secondary role as a Q-SNARE involved in endosome fusion; the protein is associated with genetic susceptibility to attention-deficit hyperactivity disorder (ADHD) (3).
Function: t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells.
Subunit Structure: Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A; this complex binds CPLX1 (PubMed:11832227). Found in a complex containing SYT1, SV2B and syntaxin-1 (By similarity). Found in a ternary complex with STX1A and VAMP8 (By similarity). Isoform 1 and isoform 2 interact with BLOC1S6 (PubMed:19546860). Interacts with CENPF (By similarity). Interacts with EQTN (By similarity). Interacts with HGS (By similarity). Interacts with KCNB1 (via N-terminus); reduces the voltage-dependent potassium channel KCNB1 activity in pancreatic beta cells (By similarity). Interacts with OTOF (By similarity). Interacts with RIMS1 (By similarity). Interacts with SNAPIN (By similarity). Interacts with STXBP6 (By similarity). Interacts with TRIM9 (By similarity). Interacts with ZDHHC13 (via ANK repeats) (By similarity). Interacts with ZDHHC17 (via ANK repeats) (PubMed:28882895, PubMed:28757145). Interacts with PLCL1 (via C2 domain) (By similarity). Associates with the BLOC-1 complex (PubMed:19546860). Interacts with PRRT2; this interaction may impair the formation of the SNARE complex (PubMed:22832103, PubMed:25915028).
Post-translational Modifications: Palmitoylated (PubMed:28757145). Cys-85 appears to be the main site, and palmitoylation is required for membrane association (By similarity).
Similarity: Belongs to the SNAP-25 family.

NCBI and Uniprot Product Information

• NCBI GI #: 1018443229
• NCBI GeneID: 6616
• NCBI Accession #: NP_001309831.1
• NCBI GenBank Nucleotide #: NM_001322902.1
• UniProt Accession #: P60880; P13795; P36974; P70557; P70558; Q53EM2; Q5U0B5; Q8IXK3; Q96FM2; B2RAU4; D3DW16; D3DW17
• Molecular Weight: Observed: 25 kDa; Predicted: 24 kDa
• NCBI Official Full Name: synaptosomal-associated protein 25 isoform SNAP25A
• NCBI Official Synonym Full Names: synaptosome associated protein 25
• NCBI Official Symbol: SNAP25
• NCBI Official Synonym Symbols: SUP; RIC4; SEC9; SNAP; CMS18; RIC-4; SNAP-25; bA416N4.2; dJ1068F16.2
• NCBI Protein Information: synaptosomal-associated protein 25
• UniProt Protein Name: Synaptosomal-associated protein 25
• Protein Family: SNAP25 homologous protein
• UniProt Gene Name: SNAP25
• UniProt Synonym Gene Names: SNAP; SNAP-25; SUP

NCBI Description

Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells.