Rabbit RBM8A Polyclonal Antibody | anti-RBM8A antibody

Anti-RBM8A Antibody

Cat. Num. AAA8307902

• Gene Names: RBM8A; TAR; Y14; RBM8; ZNRP; RBM8B; ZRNP1; BOV-1A; BOV-1B; BOV-1C; MDS014; DEL1q21.1; C1DELq21.1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Purified by Immunogen Affinity Chromatography
• Synonyms: RBM8A; Polyclonal Antibody; Anti-RBM8A Antibody; RBM8; RNA-binding protein 8A; Binder of OVCA1-1; BOV-1; RNA-binding motif protein 8A; RNA-binding protein Y14; Ribonucleoprotein RBM8A; anti-RBM8A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes endogenous levels of RBM8A protein.
• Purity/Purification: Purified by Immunogen Affinity Chromatography
• Form/Format: Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.

• Applicable Applications for anti-RBM8A antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Recombinant fusion protein of human RBM8A. The exact sequence is proprietary.
• Preparation and Storage: Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of RBM8A expression in HepG2 (A), HeLa (B), mouse heart (C), mouse kidney (D), rat liver (E) whole cell lysates.)

Related Product Information for anti-RBM8A antibody

Rabbit polyclonal antibody to RBM8A

NCBI and Uniprot Product Information

• NCBI GI #: 4826972
• NCBI GeneID: 9939
• NCBI Accession #: NP_005096.1
• NCBI GenBank Nucleotide #: NM_005105.4
• UniProt Accession #: Q9Y5S9; Q9CWZ3; Q27W01
• Molecular Weight: 19,760 Da
• NCBI Official Full Name: RNA-binding protein 8A
• NCBI Official Synonym Full Names: RNA binding motif protein 8A
• NCBI Official Symbol: RBM8A
• NCBI Official Synonym Symbols: TAR; Y14; RBM8; ZNRP; RBM8B; ZRNP1; BOV-1A; BOV-1B; BOV-1C; MDS014; DEL1q21.1; C1DELq21.1
• NCBI Protein Information: RNA-binding protein 8A; BOV-1; RNA binding motif protein 8B; RNA-binding motif protein 8A; RNA-binding protein Y14; binder of OVCA1-1; ribonucleoprotein RBM8; ribonucleoprotein RBM8A
• UniProt Protein Name: RNA-binding protein 8A
• Protein Family: RNA-binding protein
• UniProt Gene Name: RBM8A
• UniProt Synonym Gene Names: RBM8; BOV-1
• UniProt Entry Name: RBM8A_HUMAN

NCBI Description

This gene encodes a protein with a conserved RNA-binding motif. The protein is found predominantly in the nucleus, although it is also present in the cytoplasm. It is preferentially associated with mRNAs produced by splicing, including both nuclear mRNAs and newly exported cytoplasmic mRNAs. It is thought that the protein remains associated with spliced mRNAs as a tag to indicate where introns had been present, thus coupling pre- and post-mRNA splicing events. Previously, it was thought that two genes encode this protein, RBM8A and RBM8B; it is now thought that the RBM8B locus is a pseudogene. There are two alternate translation start codons with this gene, which result in two forms of the protein. An allele mutation and a low-frequency noncoding single-nucleotide polymorphism (SNP) in this gene cause thrombocytopenia-absent radius (TAR) syndrome. [provided by RefSeq, Jul 2013]

Uniprot Description

RBM8A: Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The heterodimer MAGOH-RBM8A interacts with PYM that function to enhance the translation of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Remains associated with mRNAs in the cytoplasm until the mRNAs engage the translation machinery. Its removal from cytoplasmic mRNAs requires translation initiation from EJC-bearing spliced mRNAs. Associates preferentially with mRNAs produced by splicing. Does not interact with pre-mRNAs, introns, or mRNAs produced from intronless cDNAs. Associates with both nuclear mRNAs and newly exported cytoplasmic mRNAs. Complex with MAGOH is a component of the nonsense mediated decay (NMD) pathway. Belongs to the RBM8A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Translation; RNA splicing; Spliceosome; RNA-binding

Chromosomal Location of Human Ortholog: 1q21.1

Cellular Component: nucleoplasm; cytoplasm; nuclear speck; nucleus; cytosol

Molecular Function: mRNA binding; protein binding; RNA binding; nucleotide binding

Biological Process: transcription from RNA polymerase II promoter; regulation of translation; nuclear mRNA splicing, via spliceosome; mRNA export from nucleus; RNA splicing; mRNA catabolic process, nonsense-mediated decay; gene expression; mRNA 3'-end processing; termination of RNA polymerase II transcription; regulation of alternative nuclear mRNA splicing, via spliceosome

Disease: Thrombocytopenia-absent Radius Syndrome